系统生物学的力量：从加速药物研发伙伴关系中获得的狼疮肾炎的见解。
The Power of Systems Biology: Insights on Lupus Nephritis from the Accelerating Medicines Partnership.
机构信息
Division of Rheumatology, Johns Hopkins University, 1830 East Monument Street, Suite 7500, Baltimore, MD 21205, USA.
Division of Rheumatology, Inflammation, Immunity, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA; Center for Data Sciences, Brigham and Women's Hospital, Building for Transformative Medicine, 60 Fenwood Road, Boston, MA 02115, USA; Program in Medical and Population Genetics, Broad Institute of MIT and Harvard, Cambridge, MA, USA; Centre for Genetics and Genomics Versus Arthritis, Centre for Musculoskeletal Research, Manchester Academic Health Science Centre, The University of Manchester, Oxford Road, Manchester, UK. Electronic address: https://twitter.com/soumya_boston.
出版信息
Rheum Dis Clin North Am. 2021 Aug;47(3):335-350. doi: 10.1016/j.rdc.2021.04.003. Epub 2021 Jun 16.
The Accelerating Medicines Partnership (AMP) SLE Network united resources from academic centers, government, nonprofit, and industry to accelerate discovery in lupus nephritis (LN). The AMP SLE Network developed a set of protocols for high-throughput analyses to systematically study kidney tissue, urine, and blood in LN. This article summarizes approaches and results from phase 1 of AMP SLE Network effort, including single cell RNA-seq analysis of LN kidney biopsies, cellular and proteomic studies of LN urine, and mass cytometry immunophenotyping of blood cells. This work provides a framework to guide studies of the clinical implications of active cellular/molecular pathways in LN.
加速药物研发合作组织 (AMP) 狼疮网络联合了学术中心、政府、非营利组织和工业界的资源，以加速狼疮肾炎 (LN) 的发现。AMP 狼疮网络制定了一套高通量分析方案，用于系统地研究 LN 肾组织、尿液和血液。本文总结了 AMP 狼疮网络第一阶段的工作方法和结果，包括 LN 肾活检的单细胞 RNA-seq 分析、LN 尿液的细胞和蛋白质组学研究，以及血液细胞的质谱细胞免疫表型分析。这项工作为研究 LN 中活跃的细胞/分子途径的临床意义提供了框架。
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