Department of Biomedical and Diagnostic Sciences, College of Veterinary Medicine, The University of Tennessee, Knoxville, TN, USA.
Department of Small Animal Clinical Sciences, College of Veterinary Medicine, The University of Tennessee, Knoxville, TN, USA.
Biomed Chromatogr. 2021 Dec;35(12):e5210. doi: 10.1002/bmc.5210. Epub 2021 Jul 16.
The goal of this investigation was to establish a reliable technique for the quantitation of ponazuril in limited sample volumes. Samples were extracted with chloroform and separation was achieved with a Symmetry RP column. Ultraviolet absorption was measured at 254 nm. A combination of 0.1% formic acid and acetonitrile (50:50) was used as the mobile phase. The calibration curve was linear from 0.1-25 μg/mL, with a lower limit of quantification of 0.1 μg/mL with a 100 μL sample. The precision and accuracy were well within the range set by the Food and Drug Administration and the recovery was over 95%. This technique was used to analyze ponazuril samples and found to be appropriate for pharmacokinetic studies.
本研究的目的是建立一种可靠的技术，以定量有限体积样本中的苯扎屈林。样品用氯仿提取，用 Symmetry RP 柱进行分离。在 254nm 处测量紫外吸收。采用 0.1%甲酸和乙腈（50:50）的混合物作为流动相。校准曲线在 0.1-25μg/mL 范围内呈线性，100μL 样品的定量下限为 0.1μg/mL。精密度和准确度均在食品和药物管理局规定的范围内，回收率超过 95%。该技术用于分析苯扎屈林样品，适用于药代动力学研究。
