Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
Institute of Quality and Standard for Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
BMC Genomics. 2021 Jul 3;22(1):502. doi: 10.1186/s12864-021-07811-y.
Dinotefuran (CAS No. 165252-70-0), a neonicotinoid insecticide, has been used to protect various crops against invertebrate pests and has been associated with numerous negative sublethal effects on honey bees. Long noncoding RNAs (lncRNAs) play important roles in mediating various biological and pathological processes, involving transcriptional and gene regulation. The effects of dinotefuran on lncRNA expression and lncRNA function in the honey bee brain are still obscure.
Through RNA sequencing, a comprehensive analysis of lncRNAs and mRNAs was performed following exposure to 0.01 mg/L dinotefuran for 1, 5, and 10 d. In total, 312 lncRNAs and 1341 mRNAs, 347 lncRNAs and 1458 mRNAs, and 345 lncRNAs and 1155 mRNAs were found to be differentially expressed (DE) on days 1, 5 and 10, respectively. Gene set enrichment analysis (GSEA) indicated that the dinotefuran-treated group showed enrichment in carbohydrate and protein metabolism and immune-inflammatory responses such as glycine, serine and threonine metabolism, pentose and glucuronate interconversion, and Hippo and transforming growth factor-β (TGF-β) signaling pathways. Moreover, the DE lncRNA TCONS_00086519 was shown by fluorescence in situ hybridization (FISH) to be distributed mainly in the cytoplasm, suggesting that it may serve as a competing endogenous RNA and a regulatory factor in the immune response to dinotefuran.
This study characterized the expression profile of lncRNAs upon exposure to neonicotinoid insecticides in young adult honey bees and provided a framework for further study of the role of lncRNAs in honey bee growth and the immune response.
呋虫胺（CAS 号 165252-70-0），一种新烟碱类杀虫剂，被用于保护各种作物免受无脊椎害虫侵害，并与对蜜蜂产生的许多负面亚致死效应有关。长链非编码 RNA（lncRNA）在介导各种生物和病理过程中发挥着重要作用，涉及转录和基因调控。呋虫胺对蜜蜂大脑中 lncRNA 表达和 lncRNA 功能的影响尚不清楚。
通过 RNA 测序，在暴露于 0.01mg/L 呋虫胺 1、5 和 10 天后，对 lncRNA 和 mRNA 进行了全面分析。总共发现 312 个 lncRNA 和 1341 个 mRNA、347 个 lncRNA 和 1458 个 mRNA、345 个 lncRNA 和 1155 个 mRNA 在第 1、5 和 10 天分别出现差异表达（DE）。基因集富集分析（GSEA）表明，呋虫胺处理组在碳水化合物和蛋白质代谢以及免疫炎症反应中表现出富集，如甘氨酸、丝氨酸和苏氨酸代谢、戊糖和葡萄糖醛酸转化以及 Hippo 和转化生长因子-β（TGF-β）信号通路。此外，通过荧光原位杂交（FISH）显示 DE lncRNA TCONS_00086519 主要分布在细胞质中，表明它可能作为竞争内源性 RNA 和免疫反应中对呋虫胺的调节因子。
本研究描述了在年轻成年蜜蜂中暴露于新烟碱类杀虫剂后 lncRNA 的表达谱，并为进一步研究 lncRNA 在蜜蜂生长和免疫反应中的作用提供了框架。
