Department of Medical Biotechnology, School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Biochimie. 2021 Oct;189:144-157. doi: 10.1016/j.biochi.2021.06.016. Epub 2021 Jul 1.
Because of health-promoting effects, the adaptation of skeletal muscles to exercise is considered a therapeutic strategy for metabolic complications and musculoskeletal disabilities. Myokines display many beneficial effects of different exercise modalities. Among them, irisin is known as a systemic effector that positively influences several organs. There are a few studies about the effects of irisin on skeletal muscles, and irisin prosperities need to be well-defined for being an exercise mimetic. To aim this purpose, we assessed the proteome profile of mouse skeletal muscle after eight weeks of irisin injection comparing to resistance and endurance exercise treated groups. In the current study, two-dimensional gel electrophoresis was used to evaluate the protein content of the quadriceps muscle. The results were analyzed with Image Master 2D Platinum V6 software. Differentially expressed proteins were characterized by mass spectrometry (MALDI TOF/TOF) and interpreted using protein data banks and co-expression network. Irisin increases cellular ATP content by driving its overproduction through glycolysis and oxidative phosphorylation similar to two exercise protocols and as a specific property, decreases ATP consumption through creatine kinase downregulation. It also improves the microstructural properties of quadriceps muscle by increasing fiber proteins and might induce cellular proliferation and differentiation. Network analysis of differentially expressed proteins also revealed the co-expression of Irisin precursor with structural and metabolic-related proteins. The protein alterations after irisin administration display the potential of this myokine to mimic some molecular effects of exercise, suggesting it a promising candidate to improve muscle metabolism and structure.
由于具有促进健康的作用，骨骼肌肉对运动的适应被认为是治疗代谢并发症和肌肉骨骼残疾的一种策略。肌肉因子显示出多种不同运动方式的有益作用。其中，鸢尾素被认为是一种全身性效应物，对多个器官有积极影响。有一些关于鸢尾素对骨骼肌肉影响的研究，但鸢尾素的特性需要进一步明确，才能成为一种模拟运动的物质。为了达到这个目的，我们评估了 8 周鸢尾素注射后小鼠骨骼肌的蛋白质组图谱，与抗阻运动和耐力运动治疗组进行比较。在本研究中，二维凝胶电泳用于评估股四头肌的蛋白质含量。使用 Image Master 2D Platinum V6 软件对结果进行分析。差异表达的蛋白质通过质谱（MALDI TOF/TOF）进行鉴定，并使用蛋白质数据库和共表达网络进行解释。鸢尾素通过促进糖酵解和氧化磷酸化使细胞 ATP 含量增加，与两种运动方案相似，并且通过下调肌酸激酶来降低 ATP 消耗，这是其独特的特性。它还通过增加纤维蛋白来改善股四头肌的微观结构特性，并可能诱导细胞增殖和分化。差异表达蛋白的网络分析还显示，鸢尾素前体与结构和代谢相关蛋白的共表达。鸢尾素给药后的蛋白质改变显示了这种肌肉因子模拟运动某些分子作用的潜力，表明它是改善肌肉代谢和结构的有前途的候选物质。
