School of Materials Science and Engineering, Chang'an University, Xi'an, Shaanxi, 710064, PR China.
School of Materials Science and Engineering, Chang'an University, Xi'an, Shaanxi, 710064, PR China; Engineering Research Central of Pavement Materials, Ministry of Education of PR China, Chang'an University, Xi'an, 710064, PR China.
Colloids Surf B Biointerfaces. 2021 Oct;206:111959. doi: 10.1016/j.colsurfb.2021.111959. Epub 2021 Jun 30.
Zwitterionic polymers play an attractive role in the application of stealthy nanocarriers for their excellent antifouling property. Herein, a zwitterionic nanogel with temperature sensitivity and redox-responsive degradability prepared by copolymerization of N-vinylcaprolactam (VCL) and 2-(methacryloyloxy) ethyldimethyl-(3-sulfopropyl) ammonium hydroxide (DMAPS) via aqueous precipitation polymerization. The prepared nanogels own ultra-high colloidal stability and non-specific protein adsorption resistance as a result of the incorporation of zwitterionic groups. Meanwhile, they exhibit sensitive temperature-induced swelling/collapse transition in aqueous solution and excellent redox-degradability ascribed to the presence of disulfide bonds. The nanogels loaded with anticancer drug doxorubicin (DOX) exhibit low leakage of DOX under physiological conditions (merely 23.8 % within 24 h), whereas striking release amount of DOX under reducing conditions combined with elevated temperature (93.4 % within 24 h). The measurement of cell viability showed that the cytotoxicity of blank nanogels to tumor cells (HeLa cells) was negligible, while the nanogels loaded with DOX had a prominent inhibitory impact on tumor cells.
两性离子聚合物因其出色的抗污性能，在隐形纳米载体的应用中具有吸引力。本文通过水溶液沉淀聚合，由 N-乙烯基己内酰胺（VCL）和 2-（甲基丙烯酰氧基）乙基二甲基-（3-磺丙基）氢氧化铵（DMAPS）共聚合成了一种具有温度响应性和氧化还原响应性降解性的两性离子纳米凝胶。由于引入了两性离子基团，所制备的纳米凝胶具有超高的胶体稳定性和非特异性蛋白质吸附阻力。同时，由于二硫键的存在，它们在水溶液中表现出敏感的温敏溶胀/收缩转变和优异的氧化还原降解性。载有抗癌药物阿霉素（DOX）的纳米凝胶在生理条件下（仅 24 小时内 23.8%）的 DOX 泄漏量低，而在还原条件下结合升高的温度时，DOX 的释放量显著增加（24 小时内 93.4%）。细胞活力的测量表明，空白纳米凝胶对肿瘤细胞（HeLa 细胞）的细胞毒性可以忽略不计，而载有 DOX 的纳米凝胶对肿瘤细胞具有显著的抑制作用。
