Teagasc Food Research Centre, Dublin, Ireland.
School of Veterinary Medicine, University College Dublin, Dublin 4, Ireland.
J Appl Microbiol. 2022 Jan;132(1):687-695. doi: 10.1111/jam.15204. Epub 2021 Aug 7.
This research tested the anti-Campylobacter properties of organic acids (OA), medium chain fatty acids (MCFA) and essential oils (EO) in vitro and commenced in vivo suitability testing focused on broiler performance.
Nine active compounds were tested at different concentrations and times against Campylobacter jejuni in sterile distilled water, Mueller Hinton broth and grower feed digestate (GFD). Sodium caprate (1.5%, v/v), thymol (0.25% and 2.5%, v/v), carvacrol (1.25%, v/v) and potassium sorbate (1.5%, v/v) each achieved C. jejuni reductions of ≥4.5 log CFU per ml in GFD, the matrix most representative of the broiler gut, after 60 s. Similar reductions were achieved after 60 min with lactic acid (1.25%, v/v), formic acid (3.1%, v/v), sodium caprylate (1.5%, v/v) and carvacrol (1.25%, v/v). However, in vivo these compounds adversely affected broiler performance, resulting in dimished water intake and reduced weight.
OA, MFCA and EO based compounds are effective anti-Campylobacter treatments in laboratory model studies but cannot be applied in vivo.
This study illustrates that OAs, MCFAs and EOs can achieve significant reductions in Campylobacter in vitro but identifies a major issue, inhibition of broiler performance, preventing their use in practice.
本研究旨在测试有机酸（OA）、中链脂肪酸（MCFA）和精油（EO）在体外的抗空肠弯曲杆菌特性，并开始进行体内适用性测试，重点关注肉鸡性能。
在无菌蒸馏水、Mueller Hinton 肉汤和生长饲料消化物（GFD）中，以不同浓度和时间测试了 9 种活性化合物对空肠弯曲杆菌的作用。癸酸钠（1.5%，v/v）、百里酚（0.25%和 2.5%，v/v）、香芹酚（1.25%，v/v）和山梨酸钾（1.5%，v/v）在 GFD 中均能在 60s 内使 C. jejuni 减少≥4.5 log CFU/ml，GFD 是最能代表肉鸡肠道的基质。在 60min 内，乳酸（1.25%，v/v）、甲酸（3.1%，v/v）、癸酸钠（1.5%，v/v）和香芹酚（1.25%，v/v）也能达到类似的减少效果。然而，这些化合物在体内会对肉鸡性能产生不利影响，导致饮水量减少和体重减轻。
基于 OA、MCFA 和 EO 的化合物在实验室模型研究中是有效的抗空肠弯曲杆菌治疗方法，但不能在体内应用。
本研究表明，OA、MCFA 和 EO 可以在体外显著减少空肠弯曲杆菌的数量，但也发现了一个主要问题，即抑制肉鸡性能，阻止了它们在实践中的应用。
