亚精胺和甲基乙二醛双（脒腙）在幼仓鼠肾细胞（BHK-21/C13）中的乙酰化作用
Acetylation of spermidine and methylglyoxal bis(guanylhydrazone) in baby-hamster kidney cells (BHK-21/C13).
作者信息
Wallace H M, Nuttall M E, Robinson F C
机构信息
Department of Medicine and Therapeutics, University of Aberdeen, Foresterhill, Scotland, U.K.
出版信息
Biochem J. 1988 Jul 1;253(1):223-7. doi: 10.1042/bj2530223.
Abstract
Treatment of BHK-21/C13 cells with methylglyoxal bis(guanylhydrazone) (MGBG) induced the cytosolic form of spermidine N1-acetyltransferase. It stabilized the enzyme against proteolytic degradation, but the drug did not affect the enzyme activity in vitro. MGBG was itself acetylated by BHK-21/C13 cells, but at only one-tenth the rate at which spermidine was acetylated. Acetylation occurred almost exclusively in the nuclear fraction. The product was identified as N-acetyl-MGBG by h.p.l.c., by using [3H]acetyl-CoA and [14C]MGBG as co-substrates. The results suggest that the acetylation of MGBG by BHK-21/C13 cells occurs by a different acetyltransferase enzyme from that which acetylates spermidine.
摘要
用双（胍腙）甲基乙二醛（MGBG）处理BHK - 21/C13细胞可诱导出胞质形式的亚精胺N1 - 乙酰转移酶。它使该酶稳定，抵抗蛋白水解降解，但该药物在体外不影响酶活性。MGBG本身被BHK - 21/C13细胞乙酰化，但其乙酰化速率仅为亚精胺乙酰化速率的十分之一。乙酰化几乎只发生在细胞核部分。通过使用[3H]乙酰辅酶A和[14C]MGBG作为共底物，通过高效液相色谱法将产物鉴定为N - 乙酰 - MGBG。结果表明，BHK - 21/C13细胞对MGBG的乙酰化是由一种不同于使亚精胺乙酰化的乙酰转移酶催化的。
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