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通过荧光成像和技术对整合素分子进行可视化。

Visualization of integrin molecules by fluorescence imaging and techniques.

作者信息

Cai Chen, Sun Hao, Hu Liang, Fan Zhichao

机构信息

Department of Immunology, School of Medicine, UConn Health, Farmington, 06030, USA.

Department of Medicine, University of California, San Diego, La Jolla, 92093, USA.

出版信息

Biocell. 2021;45(2):229-257. doi: 10.32604/biocell.2021.014338. Epub 2021 Feb 19.

DOI:10.32604/biocell.2021.014338
PMID:34219865
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8249084/
Abstract

Integrin molecules are transmembrane αβ heterodimers involved in cell adhesion, trafficking, and signaling. Upon activation, integrins undergo dynamic conformational changes that regulate their affinity to ligands. The physiological functions and activation mechanisms of integrins have been heavily discussed in previous studies and reviews, but the fluorescence imaging techniques -which are powerful tools for biological studies- have not. Here we review the fluorescence labeling methods, imaging techniques, as well as Förster resonance energy transfer assays used to study integrin expression, localization, activation, and functions.

摘要

整合素分子是参与细胞黏附、运输和信号传导的跨膜αβ异二聚体。激活后，整合素会发生动态构象变化，从而调节其对配体的亲和力。整合素的生理功能和激活机制在以往的研究和综述中已有大量讨论，但荧光成像技术（生物学研究的强大工具）却未被充分探讨。在此，我们综述了用于研究整合素表达、定位、激活和功能的荧光标记方法、成像技术以及荧光共振能量转移测定法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd33/8249084/dfafe67d2a87/nihms-1661428-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd33/8249084/d0bd4d2dde5e/nihms-1661428-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd33/8249084/ea5e0c723af0/nihms-1661428-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd33/8249084/dfafe67d2a87/nihms-1661428-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd33/8249084/d0bd4d2dde5e/nihms-1661428-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd33/8249084/ea5e0c723af0/nihms-1661428-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd33/8249084/dfafe67d2a87/nihms-1661428-f0003.jpg

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Seeing around corners: Cells solve mazes and respond at a distance using attractant breakdown.见微知著：细胞利用分解的趋化因子解决迷津并远距离响应。
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ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells.
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