Sagor G H M, Inoue Masataka, Kusano Tomonobu, Berberich Thomas
Plant Molecular Genetics Laboratory, Department of Genetics & Plant Breeding, Bangladesh Agricultural University, Mymensingh, 2202 Bangladesh.
Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba, Sendai, 980-8577 Japan.
Physiol Mol Biol Plants. 2021 Jun;27(6):1353-1359. doi: 10.1007/s12298-021-01006-1. Epub 2021 May 28.
Polyamine levels are controlled by biosynthesis, intra- and inter-cellular flux by the respective transporters, and catabolism. The catabolism is catalyzed by two groups of enzymes. One is copper-containing amine oxidases and the other is polyamine oxidases (PAOs). In , seven genes exist and they are termed as to . However, their physiological function has not been elucidated yet. Here, we examined the expressional changes of seven genes upon abiotic and oxidative stress, phytohormone, and exogenous polyamines application. The transcript of extracellular polyamine oxidase and are strongly induced upon wounding, drought, salinity, oxidative stress (HO), and exogenous application of jasmonic acid, spermidine, spermine, thermospermine and negatively regulated upon indole acetic acid, isopentenyl adenine (iPT), gibberellic acid (GA), abscisic acid; is to iPT, GA and all polyamines; and are mildly responsive to heat, cold, oxidative stress. These results suggest that polyamine oxidase encoding extracellular enzyme may play a pivotal role during exogenous stimulus to protect the plant cell.
The online version contains supplementary material available at 10.1007/s12298-021-01006-1.
多胺水平受生物合成、各自转运蛋白介导的细胞内和细胞间通量以及分解代谢的控制。分解代谢由两组酶催化。一组是含铜胺氧化酶，另一组是多胺氧化酶（PAO）。在[具体物种]中，存在七个[基因名称]基因，它们被命名为[具体基因名称1]至[具体基因名称7]。然而，它们的生理功能尚未阐明。在此，我们研究了七个[基因名称]基因在非生物和氧化应激、植物激素以及外源多胺处理后的表达变化。细胞外多胺氧化酶[具体基因名称1]和[具体基因名称2]的转录本在伤口、干旱、盐度、氧化应激（H₂O₂）以及茉莉酸、亚精胺、精胺、热精胺的外源处理后强烈诱导，而在吲哚乙酸、异戊烯基腺嘌呤（iPT）、赤霉素（GA）、脱落酸处理后受到负调控；[具体基因名称3]对iPT、GA和所有多胺有反应；[具体基因名称4]和[具体基因名称5]对热、冷、氧化应激有轻微反应。这些结果表明，编码细胞外酶的多胺氧化酶可能在外部刺激期间发挥关键作用以保护植物细胞。
在线版本包含可在10.1007/s12298-021-01006-1获取的补充材料。
