Sagor G H M, Kusano Tomonobu, Berberich Thomas
a Laboratory Center , Senckenberg Biodiversity and Climate Research Center , Frankfurt am Main , Germany.
b Graduate School of Life Sciences , Tohoku University , Sendai , Japan.
Plant Signal Behav. 2017 Aug 3;12(8):e1359456. doi: 10.1080/15592324.2017.1359456. Epub 2017 Aug 8.
Polyamines (PA) in plant play roles in growth and development and in responses to environmental stresses. The family of polyamine oxidases (PAO) contributes to a balanced homeostasis of PAs catalyzing two different reactions, terminal catabolic (TC) and back-conversion (BC) pathway, in PA catabolism. From the seven PAOs encoded by the rice genome (OsPAO1 - OsPAO7) OsPAO6 could so far not be characterized due to failure in obtaining the coding cDNA based on accessions in the genomic databases. We report cloning and characterization of the correct OsPAO6 cDNA with a length of 1,742 bp. The 1,491 bp long open reading frame codes for a 497-amino acid protein from nine exons. The protein which has 92% identity to OsPAO7 localizes to plasma membrane.
植物中的多胺（PA）在生长发育以及对环境胁迫的响应中发挥作用。多胺氧化酶（PAO）家族通过催化PA分解代谢中的两种不同反应，即末端分解代谢（TC）和回转化（BC）途径，有助于维持PA的平衡稳态。水稻基因组编码了7种PAO（OsPAO1 - OsPAO7），由于基于基因组数据库中的登录号未能获得编码cDNA，到目前为止OsPAO6的特性仍未明确。我们报道了长度为1742 bp的正确的OsPAO6 cDNA的克隆及特性分析。1491 bp长的开放阅读框由9个外显子编码一个497个氨基酸的蛋白质。该蛋白质与OsPAO7有92%的同一性，定位于质膜。
