Rodionov A V
Bioorg Khim. 1988 May;14(5):581-8.
Programmes for automatic analysis of protein and peptide hydrolysates and physiological fluids in a single system of sodium citrate buffers are proposed. Retention time values of 59 ninhydrin-positive substances, including nonprotein amino acids, amino sugars (muramic acid, glucosamine, galactosamine, mannosamine and fucosamine) and corresponding aminopolyols, as well as three cysteine derivatives, viz. cysteic acid, S-carboxymethylcysteine and S-2-(pyridyl-4)ethylcysteine, have been determined. Several examples illustrate an increase of the resolution simply by change of the temperature programme of the column and (or) elution time.
提出了在柠檬酸钠缓冲液单一系统中对蛋白质和肽水解产物以及生理流体进行自动分析的程序。已测定了59种茚三酮阳性物质的保留时间值，这些物质包括非蛋白质氨基酸、氨基糖（胞壁酸、葡萄糖胺、半乳糖胺、甘露糖胺和岩藻糖胺）及相应的氨基多元醇，还有三种半胱氨酸衍生物，即半胱磺酸、S-羧甲基半胱氨酸和S-2-(吡啶-4)乙基半胱氨酸。几个例子表明，仅通过改变柱温程序和（或）洗脱时间就能提高分离度。
