Veremeĭchenko S N
Mikrobiol Z. 1996 Mar-Apr;58(2):70-5.
A method for analysis of phosphoethanolamine in hydrolysates of the fractions structural parts of lipopolysaccharide macromolecule on the column with cation-exchange resin Ostion LG ANB (CSFR) in the system of step gradient of sodium-citrate buffers is suggested, optimal conditions for acidic hydrolysis of polysaccharide-standard were chosen to determine phosphoethanolamine and other amino compounds contained in it. The conditions for chromatography analysis of phosphoethanolamine together with aminosugars and amino acids have been developed. Using the suggested procedure the presence of phosphoethanolamine was found in preparations of lipid A hydrolysates and core oligosaccharide isolated from some lipopolysaccharide strains of Pseudomonas fluorescens.
提出了一种在柠檬酸钠缓冲液分步梯度系统中，用阳离子交换树脂Ostion LG ANB（CSFR）柱分析脂多糖大分子结构部分水解产物中磷酸乙醇胺的方法，选择了多糖标准品酸性水解的最佳条件以测定其中所含的磷酸乙醇胺和其他氨基化合物。已开发出磷酸乙醇胺与氨基糖和氨基酸一起进行色谱分析的条件。使用所建议的方法，在从荧光假单胞菌的一些脂多糖菌株中分离出的脂质A水解产物和核心寡糖制剂中发现了磷酸乙醇胺的存在。
