Adachi K, Okumura M, Tanimoto M, Morishima Y, Ohno R, Saito H
First Department of Internal Medicine, Nagoya University School of Medicine, Japan.
J Lab Clin Med. 1988 Jan;111(1):125-32.
We have examined the immunophenotype and genotype of leukemic cells from 20 patients with the blastic phase of chronic myelogenous leukemia (CML), which is known to arise from a pluripotential hematopoietic stem cell. The phenotypic analysis of surface antigens with a panel of lineage-specific monoclonal antibodies revealed that six of 20 cases expressed phenotypes of lymphoid blastic transformation with pre-B cell markers. One of these cases was shown to coexpress cluster designation 2 of T cell marker on the same cells by two-color immunofluorescence analysis. Eight cases, including two cases that also had a megakaryocytic component, showed phenotypes expressing differentiation antigens of myeloid series. Three expressed a phenotype of megakaryoblastic transformation. The remaining three cases had no surface marker characteristic of any cellular lineage. The genotypic analysis by Southern blot hybridization showed that immunoglobulin heavy chain genes were rearranged in all of six lymphoid blastic transformations and that rearrangement of a T cell receptor beta chain gene was present in only one lymphoid blastic transformation that had no T cell surface marker. DNA samples in all cases of nonlymphoid blastic transformation were retained in the germ line configuration for both immunoglobulin and T cell receptor genes.(ABSTRACT TRUNCATED AT 250 WORDS)
我们检测了20例慢性粒细胞白血病（CML）急变期患者白血病细胞的免疫表型和基因型，已知该急变期源自多能造血干细胞。用一组谱系特异性单克隆抗体对表面抗原进行表型分析显示，20例中有6例表达带有前B细胞标志物的淋巴母细胞转化表型。通过双色免疫荧光分析表明，其中1例在同一细胞上共表达T细胞标志物的CD2。包括2例也有巨核细胞成分的8例，表现出表达髓系系列分化抗原的表型。3例表达巨核母细胞转化表型。其余3例没有任何细胞谱系的表面标志物特征。通过Southern印迹杂交进行的基因分析显示，在所有6例淋巴母细胞转化中免疫球蛋白重链基因发生重排，而在1例没有T细胞表面标志物的淋巴母细胞转化中仅存在T细胞受体β链基因重排。所有非淋巴母细胞转化病例的DNA样本中，免疫球蛋白和T细胞受体基因均保持种系构型。（摘要截短于250字）
