Greenberg J M, Quertermous T, Seidman J G, Kersey J H
J Immunol. 1986 Sep 15;137(6):2043-9.
Rearrangement of germ-line genes coding for T and B cell antigen receptor molecules is an early event in lymphoid development which eventually leads to the generation of clonal diversity in receptor-positive lymphocytes. Three T cell-associated rearranging genes have been described. Two, T alpha and T beta, code for the two polypeptide chains that form the T cell receptor heterodimer. The function of the third gene, the gamma-gene (T gamma), is not known. To learn more about the behavior of T gamma during lymphoid ontogeny, we compared rearrangement of T gamma and T beta genes in leukemic cells arrested at varied stages of lymphoid and myeloid development. We analyzed 38 fresh cell lines and 15 established cell lines from a total of 53 leukemic patients. Cells were immunophenotyped with a panel of monoclonal antibodies recognizing T-, B-, or myeloid-associated surface markers. Sixteen T-lineage cases were studied; 15 displayed both T beta and T gamma rearrangements. The exception (germ-line for T beta and T gamma) was an immature CD2(T11)+, CD3(T3)-, CD7(3A1)+, CD1(T6)+, CD5(T101)+ phenotype. Fourteen non-T non-B leukemias were analyzed; eight were germ-line for both T beta and T gamma, four had rearrangements involving both T beta and T gamma, and two were germ-line for T beta and rearranged to T gamma. Four cases with acute biphenotypic leukemia were studied; two had rearrangements of T beta and T gamma, and two were germ-line for both genes. Cells from nonlymphocytic leukemias were studied in 19 cases. All were found to be germ-line for both T beta and T gamma. Fifty-one of 53 genomic DNA samples were concordant for T gamma and T beta rearrangement. These results indicate that rearrangement of T gamma can occur in leukemic cells of B cell as well as T cell precursor origin, as has been reported previously for T beta.
编码T细胞和B细胞抗原受体分子的种系基因重排是淋巴细胞发育过程中的早期事件，最终导致受体阳性淋巴细胞产生克隆多样性。已描述了三个与T细胞相关的重排基因。其中两个，Tα和Tβ，编码构成T细胞受体异二聚体的两条多肽链。第三个基因，γ基因（Tγ）的功能尚不清楚。为了更多地了解Tγ在淋巴细胞个体发育过程中的行为，我们比较了处于淋巴细胞和髓细胞发育不同阶段的白血病细胞中Tγ和Tβ基因的重排情况。我们分析了来自53例白血病患者的38个新鲜细胞系和15个已建立的细胞系。用一组识别T细胞、B细胞或髓细胞相关表面标志物的单克隆抗体对细胞进行免疫表型分析。研究了16例T系病例；15例同时出现Tβ和Tγ重排。例外情况（Tβ和Tγ为种系）是一种不成熟的CD2（T11）+、CD3（T3）-、CD7（3A1）+、CD1（T6）+、CD5（T101）+表型。分析了14例非T非B白血病；8例Tβ和Tγ均为种系，4例同时涉及Tβ和Tγ重排，2例Tβ为种系而重排至Tγ。研究了4例急性双表型白血病病例；2例有Tβ和Tγ重排，2例两个基因均为种系。研究了19例非淋巴细胞白血病的细胞。发现所有细胞的Tβ和Tγ均为种系。53个基因组DNA样本中有51个Tγ和Tβ重排结果一致。这些结果表明，Tγ重排可发生在B细胞以及T细胞前体来源的白血病细胞中，正如先前报道的Tβ重排情况一样。
