Alka Sadiya, Shahir Shafinaz, Ibrahim Norahim, Rahmad Norasfaliza, Haliba Norhazalina, Abd Manan Fazilah
Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, 81310 Skudai, Johor Malaysia.
Agro-Biotechnology Institute, National Institutes of Biotechnology Malaysia (NIBM), c/o MARDI Headquater, 43400 Serdang, Selangor Malaysia.
3 Biotech. 2021 Jul;11(7):336. doi: 10.1007/s13205-021-02864-y. Epub 2021 Jun 16.
Arsenic (As) is an increasing threat across the globe, widely known as a non-threshold carcinogen, and it is reaching harmful values in several areas of the world. In this study, the effect of plant growth promoting bacteria () on inorganic arsenic (Arsenate) phytoremediation by plants was investigated through histological analysis and proteome profiling of the plants. Two-dimensional gel electrophoresis and transmission electron microscopy were used to conduct the proteome and histological analysis. When arsenic-treated cells were compared to untreated cells, substantial changes were found (1) severely altered the morphology of the cells, intensely disturbed; (2) the cell wall was thicker; (3) drastically changed the cytoplasm, the cells were polygonal in shape, different in size (scattered), and relatively dense. Compared to the control group, the ultra-structure of the root cells of the control group revealed intact cytoplasm, vacuole, and cell wall under exposure to As + bacteria that had a minor effect on the cell form. To further understand As + bacteria interaction, proteome profiling of the root cell was analyzed. The As-induced oxidative stress enrichment was confirmed by the up-regulation of tubulin, nucleoside diphosphate kinase, and major allergen during As + bacteria exposure It was observed that the profusion of proteins involved in defence, protein biogenesis, signaling, photosynthesis, nucleoside and energy metabolism was greater in As + bacteria as compared to the rooting out of As only. Overall, it can be obviously seen that the current study demonstrates the effectiveness of phytoremediation by on proteins involved and responsive pathways in dealing with As toxicity in plant.
砷（As）在全球范围内构成的威胁日益增加，它作为一种无阈值致癌物广为人知，并且在世界上多个地区已达到有害值。在本研究中，通过对蜈蚣草植物进行组织学分析和蛋白质组分析，研究了植物促生细菌对蜈蚣草对无机砷（砷酸盐）植物修复的影响。利用二维凝胶电泳和透射电子显微镜进行蛋白质组和组织学分析。将经砷处理的细胞与未处理的细胞进行比较时，发现了显著变化：（1）严重改变了细胞形态，严重扰乱；（2）细胞壁变厚；（3）细胞质发生剧烈变化，细胞呈多边形，大小各异（分散），且相对致密。与对照组相比，对照组根细胞的超微结构显示，在暴露于砷+细菌的情况下，细胞质、液泡和细胞壁完整，对细胞形态影响较小。为了进一步了解砷+细菌的相互作用，对根细胞的蛋白质组进行了分析。在砷+细菌暴露期间，微管蛋白、核苷二磷酸激酶和主要变应原的上调证实了砷诱导的氧化应激富集。观察到，与仅去除砷相比，砷+细菌中参与防御、蛋白质生物合成、信号传导、光合作用、核苷和能量代谢的蛋白质数量更多。总体而言，目前的研究显然证明了蜈蚣草在处理砷毒性时对相关蛋白质和反应途径进行植物修复的有效性。
