Department of Food Sciences and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Food Hygiene, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Arch Razi Inst. 2021 Jul;76(2):231-241. doi: 10.22092/ari.2020.342066.1450. Epub 2021 Jul 1.
This study aimed to investigate the fungal species isolation and confirmation by the Multiplex PCR method in aquatic fish. Evaluation of the inhibitory effect of nano-essential oils of Carum copticum on isolated fungal species was also conducted in this study. The PCR results showed that 3 out of 5 samples were diagnosed with Fusarium solani, and two of them were positive for Saprolegnia. Moreover, in 0.1% of the females' nanoparticles, one peak appeared that showed a particle with an average diameter of 360 nm, and two nanoparticles showed a peak with a mean diameter of 242 nm. The results of minimum inhibitory concentrations (MIC) and minimum fungicidal concentrations (MFC) showed that 0.01% nano essential oil had 0.08 and 0.07 mg/ml MIC values against Fusarium solani and Saprolegnia, respectively. Gram/ml was on the growth of Fusarium solani species. The essential oils of female plants had an MIC of 0.07 in 0.1% essential oil and 0.03 mg/ml in 0.01% essential oil in Saprolegnia. Furthermore, in the case of 0.1% nano essential oil, the results showed the MIC values of 0.04 and 0.03 mg/ml against Fusarium solani and Saprolegnia, respectively. The MFC values of 0.1% nano essential oil were 0.1 and 0.07 mg/ml against Fusarium solani and Saprolegnia,respectively. It was not found on Fusarium and Saprolegnia. Overall, the results of this study using PCR for direct detection showed that 70% and 50% of the samples were Fusarium solani and Saprolegnia positive, respectively; therefore, the PCR was an efficient method for the detection of fungi. According to the results of nano-essential oil (0.1%) of females, this nano-essence had a strong inhibitory effect on Fusarium solani and Saprolegnia.
本研究旨在通过多重 PCR 方法调查水生鱼类的真菌物种分离和确认。本研究还评估了 Carum copticum 纳米精油对分离真菌的抑制作用。PCR 结果显示，5 个样本中有 3 个被诊断为尖孢镰刀菌，其中 2 个为水霉阳性。此外，在 0.1%的女性纳米粒子中，出现了一个峰值，显示出一个平均直径为 360nm 的粒子，两个纳米粒子显示出一个平均直径为 242nm 的峰值。最小抑菌浓度（MIC）和最小杀菌浓度（MFC）的结果表明，0.01%纳米精油对尖孢镰刀菌和水霉的 MIC 值分别为 0.08 和 0.07mg/ml。革兰氏阳性菌对尖孢镰刀菌的生长有抑制作用。0.01%精油中女性植物精油的 MIC 值为 0.07，0.07mg/ml。此外，在 0.1%纳米精油的情况下，MIC 值分别为 0.04 和 0.03mg/ml，对尖孢镰刀菌和水霉有效。0.1%纳米精油的 MFC 值分别为 0.07 和 0.03mg/ml，对尖孢镰刀菌和水霉有效。对尖孢镰刀菌和水霉均未检出。总的来说，本研究使用 PCR 进行直接检测的结果表明，70%和 50%的样本分别为尖孢镰刀菌和水霉阳性；因此，PCR 是一种有效的真菌检测方法。根据女性的纳米精油（0.1%）的结果，这种纳米精油对尖孢镰刀菌和水霉有很强的抑制作用。
