半乳糖凝集素-1对人单核细胞衍生树突状细胞免疫调节特性的影响。
Effects of galectin-1 on immunomodulatory properties of human monocyte-derived dendritic cells.
作者信息
Džopalić Tanja, Kostić Miloš, Kostić Milena, Marjanović Goran, Guzina Jelena, Jurišić Vladimir, Božić Nedeljković Biljana
机构信息
Department of Immunology, Medical Faculty, University of Niš, Niš, Serbia.
Faculty of Biology, Institute for Physiology and Biochemistry "Ivan Djaja", University of Belgrade, Belgrade, Serbia.
出版信息
Growth Factors. 2020 Dec;38(5-6):235-246. doi: 10.1080/08977194.2021.1947267. Epub 2021 Jul 5.
Our study aimed to evaluate the effects of Gal-1 in dose depending manner on maturation and immunomodulatory properties of monocyte-derived (Mo) DCs . The effects were analyzed by monitoring their phenotypic characteristics, cytokine profile, and the ability to direct the immune response in the co-culture with allogeneic CD4T cells. Gal-1 reduced the expression of CD80 and CD86 molecules on MoDCs compared to untreated MoDCs. Gal-1 at concentrations of 1 and 6 μg/mL significantly reduced IL-12 production, while the concentration of 3 μg/mL led to its significant increase. Gal-1 in all concentrations induced a significant increase in the production of IL-10. Treatment of MoDCs with 3 and 6 μg/mL of Gal-1 stimulated the production of IL-2 and IFN-γ in the co-culture with CD4T lymphocytes. This study demonstrated a dual immunomodulatory effect of Gal-1 on MoDCs in terms of immune stimulation and immune suppression, depending on the applied concentration.
我们的研究旨在评估不同剂量的Gal-1对单核细胞来源的（Mo）树突状细胞（DCs）成熟及免疫调节特性的影响。通过监测其表型特征、细胞因子谱以及在与同种异体CD4T细胞共培养中指导免疫反应的能力来分析这些影响。与未处理的MoDCs相比，Gal-1降低了MoDCs上CD80和CD86分子的表达。浓度为1和6μg/mL的Gal-1显著降低了IL-12的产生，而3μg/mL的浓度则导致其显著增加。所有浓度的Gal-1均诱导IL-10产生显著增加。用3和6μg/mL的Gal-1处理MoDCs可刺激其在与CD4T淋巴细胞共培养时产生IL-2和IFN-γ。这项研究表明，根据所应用的浓度，Gal-1对MoDCs具有免疫刺激和免疫抑制方面的双重免疫调节作用。
Zotero 插件