Department of Biochemistry, School of Life Sciences, University of KwaZulu-Natal (Westville Campus), Durban, 4000, South Africa; Department of Pharmacology, University of the Free State, Bloemfontein, 9300, South Africa.
Department of Biochemistry, School of Life Sciences, University of KwaZulu-Natal (Westville Campus), Durban, 4000, South Africa.
J Ethnopharmacol. 2021 Oct 28;279:114390. doi: 10.1016/j.jep.2021.114390. Epub 2021 Jul 2.
Raffia palm (Raphia hookeri G. Mann & H. Wendl) wine (RPW) is a natural beverage obtained from the R. hookeri consumed for refreshment and medicinal purposes. For medicinal purposes, it is used singly or as macerating agent for other medicinal plants for the treatment of several diseases.
This study investigates the effect of Raffia palm wine on dysregulated lipid metabolic pathways in testicular tissues of type 2 diabetic (T2D) rats.
Raffia palm wine (150 and 300 mg/kg bodyweight) was administered to two T2D groups respectively, another T2D group was not administered treatment and served as negative control, while metformin served as the standard drug. After 6 weeks of treatment, the rats were sacrificed, and the testes collected. After weighing, the organs were homogenized in 20% methanol/ethanol and centrifuged at 20,000 g to extract the lipid metabolites.
GC-MS analysis of the supernatants revealed an alteration of the metabolites on induction of T2D, with concomitant generation of 10 metabolites. Raffia palm wine inhibited the T2D-generated metabolites while replenishing cholesterol and squalene levels, with concomitant generation of 7 and 8 metabolites for low and high dose treatment respectively. Pathway enrichment analysis of the metabolites revealed a decreased level of steroid biosynthesis and increased level of fatty acid biosynthesis. Raffia palm wine inactivated glycerolipid, fatty acid, and arachidonic acid metabolisms, fatty acid biosynthesis and fatty acid elongation in mitochondria pathways, and activated pathways for plasmalogen synthesis, mitochondrial beta-oxidation of long chain saturated fatty acids.
The replenishment and generation of these metabolites and additional ones as well as activation of pathways involved in energy generation, phospholipids, antioxidant activity, steroidogenesis and spermatogenesis suggest a therapeutic effect of Raffia palm wine against hyperglycemic-induced testicular dysfunction.
拉菲棕榈（Raphia hookeri G. Mann & H. Wendl）酒（RPW）是一种从拉菲棕榈中提取的天然饮料，用于提神和药用。在药用方面，它可单独使用或作为浸渍剂用于其他药用植物，以治疗多种疾病。
本研究旨在探讨拉菲棕榈酒对 2 型糖尿病（T2D）大鼠睾丸组织中脂质代谢紊乱途径的影响。
将拉菲棕榈酒（150 和 300mg/kg 体重）分别给予两组 T2D 大鼠，另一组 T2D 大鼠不给任何治疗，作为阴性对照，而二甲双胍作为标准药物。治疗 6 周后，处死大鼠，收集睾丸。称重后，将器官在 20%甲醇/乙醇中匀浆，以 20,000g 离心提取脂质代谢物。
上清液的 GC-MS 分析显示，T2D 诱导后代谢物发生变化，同时产生 10 种代谢物。拉菲棕榈酒抑制了 T2D 产生的代谢物，同时补充了胆固醇和角鲨烯水平，低剂量和高剂量治疗分别产生了 7 种和 8 种代谢物。代谢物的通路富集分析显示，甾体生物合成水平降低，脂肪酸生物合成水平升高。拉菲棕榈酒使甘油磷脂、脂肪酸和花生四烯酸代谢、脂肪酸生物合成和线粒体中的脂肪酸延长代谢、质膜合成、长链饱和脂肪酸的线粒体β-氧化途径失活，并激活了途径。
这些代谢物的补充和产生以及参与能量生成、磷脂、抗氧化活性、甾体生成和精子发生的途径的激活表明，拉菲棕榈酒对高血糖引起的睾丸功能障碍具有治疗作用。
