Dipartimento di Medicina Sperimentale e Clinica, Sezione Scienze Fisiologiche, Università Degli Studi di Firenze, Viale G.B. Morgagni 63, Firenze, 50134, Italy.
Dipartimento di Medicina Sperimentale e Clinica, Sezione Scienze Fisiologiche, Università Degli Studi di Firenze, Viale G.B. Morgagni 63, Firenze, 50134, Italy.
Respir Physiol Neurobiol. 2021 Nov;293:103736. doi: 10.1016/j.resp.2021.103736. Epub 2021 Jul 3.
The preBötzinger complex (preBötC) is a medullary area essential for normal breathing and widely recognized as necessary and sufficient to generate the inspiratory phase of respiration. It has been studied mainly in rodents. Here we report the main results of our studies revealing the characteristics of the rabbit preBötC identified by means of neuronal recordings, D,L-homocysteic acid microinjections and histological controls. A crucial role in the respiratory rhythmogenesis within this neural substrate is played by excitatory amino acids, but also GABA and glycine display important contributions. Increases in respiratory frequency are induced by microinjections of neurokinins, somatostatin as well by serotonin (5-HT) through an action on 5-HT and 5-HT receptors or the disinhibition of a GABAergic circuit. Respiratory depression is observed in response to microinjections of the μ-opioid receptor agonist DAMGO. Our results show similarities and differences with the rodent preBötC and emphasize the importance of comparative studies on the mechanisms underlying respiratory rhythmogenesis in different animal species.
preBötzinger 复合体（preBötC）是一个对正常呼吸至关重要的延髓区域，被广泛认为是产生呼吸吸气相所必需且充分的。它主要在啮齿动物中进行了研究。在这里，我们报告了通过神经元记录、D,L-同型半胱氨酸微注射和组织学对照来鉴定兔 preBötC 的主要研究结果。在这个神经基质中的呼吸节律发生中，兴奋性氨基酸起着关键作用，但 GABA 和甘氨酸也显示出重要的贡献。神经激肽、生长抑素和 5-羟色胺（5-HT）的微注射会引起呼吸频率的增加，这是通过作用于 5-HT 和 5-HT 受体或抑制 GABA 能回路来实现的。微注射μ-阿片受体激动剂 DAMGO 会导致呼吸抑制。我们的结果显示出与啮齿动物 preBötC 的相似性和差异性，并强调了在不同动物物种中进行呼吸节律发生机制的比较研究的重要性。
