Marsh J C, Blackett N M
Exp Hematol. 1978 Feb;6(2):135-40.
A direct method of assay of the 'granulocytic repopulating ability' (GRA) of bone marrow in normal or treated mice, which utilizes determinations of total bone marrow cellularity and peroxidase positive cells, is described. Ten days after transplantation of normal femoral marrow, the total number of marrow granulocytes in the recipients is proportional to the marrow dose in the range 0.01 to 0.03 of a femur. Above 0.01 femur the proportion of granulocytes is constant at about 60% so there is a high correlation between total cellularity and total granulocytes. Assay of erythroid repopulating ability can be performed in the same animals. Using the GRA assay, the results for the dose response curve for cyclophosphamide, and the relationship of GRA to erythroid repopulating ability (ERA) and spleen colony forming cells, are similar to those obtained using the endotoxin technique. The simplicity of the GRA technique enhances the feasibility of multiple simultaneous hematopoietic precursor cell assays.
本文描述了一种直接测定正常或经处理小鼠骨髓“粒细胞再增殖能力”（GRA）的方法，该方法利用全骨髓细胞计数和过氧化物酶阳性细胞的测定。正常股骨骨髓移植10天后，受体骨髓粒细胞总数与0.01至0.03根股骨范围内的骨髓剂量成正比。超过0.01根股骨时，粒细胞比例恒定在约60%，因此全细胞计数与总粒细胞数之间存在高度相关性。红细胞再增殖能力的测定可在同一动物中进行。使用GRA测定法，环磷酰胺剂量反应曲线的结果，以及GRA与红细胞再增殖能力（ERA）和脾集落形成细胞的关系，与使用内毒素技术获得的结果相似。GRA技术的简单性提高了同时进行多种造血前体细胞测定的可行性。
