Xu Hui, Zhou Wenjing, Zhang Fan, Wu Linhui, Li Juan, Ma Tongtong, Cao Tong, Lian Chaoqun, Xia Jun, Wang Peter, Ma Jia, Li Yuyun
Department of Laboratory Medicine, School of Laboratory Medicine, Bengbu Medical College, Bengbu, Anhui, 233030, China.
Bengbu Medical College Key Laboratory of Cancer Research and Clinical Laboratory Diagnosis, Bengbu Medical College, Bengbu, Anhui, 233030, China.
Cell Death Discov. 2021 Jun 21;7(1):168. doi: 10.1038/s41420-021-00537-6.
PDS5B (precocious dissociation of sisters 5B) plays a pivotal role in carcinogenesis and progression. However, the biological functions of PDS5B in lung cancer and its underlying mechanisms are not fully elucidated. In the present study, we used MTT assays, wound-healing assays, and transwell migration and invasion approach to examine the cell viability, migration, and invasion of non-small cell lung cancer (NSCLC) cells after PDS5B modulation. Moreover, we investigated the function of PDS5B overexpression in vivo. Furthermore, we detected the expression of PDS5B in tissue samples of lung cancer patients by immunohistochemical study. We found that upregulation of PDS5B repressed cell viability, migration, and invasion in NSCLC cells, whereas downregulation of PDS5B had the opposite effects. We also observed that PDS5B overexpression retarded tumor growth in nude mice. Notably, PDS5B positively regulated LATS1 expression in NSCLC cells. Strikingly, low expression of PDS5B was associated with lymph node metastasis in lung cancer patients. Our findings suggest that PDS5B might be a therapeutic target for lung cancer.
姐妹染色单体早熟分离蛋白5B（PDS5B）在肿瘤发生和进展过程中起着关键作用。然而，PDS5B在肺癌中的生物学功能及其潜在机制尚未完全阐明。在本研究中，我们采用MTT法、伤口愈合试验以及Transwell迁移和侵袭实验，检测PDS5B调控后非小细胞肺癌（NSCLC）细胞的活力、迁移和侵袭能力。此外，我们还研究了PDS5B过表达在体内的功能。此外，我们通过免疫组织化学研究检测了肺癌患者组织样本中PDS5B的表达。我们发现，上调PDS5B可抑制NSCLC细胞的活力、迁移和侵袭，而下调PDS5B则产生相反的效果。我们还观察到，PDS5B过表达可抑制裸鼠肿瘤生长。值得注意的是，PDS5B在NSCLC细胞中正向调节LATS1的表达。令人惊讶的是，PDS5B低表达与肺癌患者的淋巴结转移有关。我们的研究结果表明，PDS5B可能是肺癌的一个治疗靶点。
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