Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, Jiangsu, P.R. China.
FEMS Microbiol Lett. 2021 Jul 20;368(14). doi: 10.1093/femsle/fnab088.
The activity of mitochondrial pyruvate carrier (MPC) can be modulated to regulate intracellular metabolism under different culture conditions. In Ganoderma lucidum, the role of MPC in regulating carbon sources remains unknown. By knocking down MPC genes (MPC1 and MPC2), this research found that the loss of MPC increased the growth rate of G. lucidum by ~30% in a medium with wood chips as a carbon source. Then cellulase and laccase activities were tested. Endoglucanase and laccase activity increased by ~50% and ~35%, respectively, in MPC knockdown mutants compared with that in the wild type strain. Finally, the expression levels of genes related to glycolysis were assayed, and the transcription levels of these enzymes were found to be increased by ~250% compared with the wild type strain. In conclusion, the regulation of intracellular metabolism by MPC provides a new way to improve the use of nondominant carbon sources such as lignocellulose.
线粒体丙酮酸载体 (MPC) 的活性可以在不同的培养条件下进行调节，以调节细胞内代谢。在灵芝中，MPC 在调节碳源方面的作用尚不清楚。通过敲除 MPC 基因 (MPC1 和 MPC2)，本研究发现，在以木屑作为碳源的培养基中，MPC 的缺失使灵芝的生长速度提高了约 30%。然后测试了纤维素酶和漆酶的活性。与野生型菌株相比，MPC 敲低突变体中的内切葡聚糖酶和漆酶活性分别提高了约 50%和 35%。最后，测定了与糖酵解相关的基因的表达水平，发现这些酶的转录水平比野生型菌株提高了约 250%。总之，MPC 对细胞内代谢的调节为提高木质纤维素等非优势碳源的利用提供了一种新方法。
