Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture and Rural Affairs; Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, PR China.
Commun Biol. 2024 Apr 17;7(1):466. doi: 10.1038/s42003-024-06180-y.
Cellulose is an important abundant renewable resource on Earth, and the microbial cellulose utilization mechanism has attracted extensive attention. Recently, some signalling molecules have been found to regulate cellulose utilization and the discovery of underlying signals has recently attracted extensive attention. In this paper, we found that the hydrogen sulfide (HS) concentration under cellulose culture condition increased to approximately 2.3-fold compared with that under glucose culture condition in Ganoderma lucidum. Further evidence shown that cellulase activities of G. lucidum were improved by 18.2-27.6% through increasing HS concentration. Then, we observed that the carbon repressor CreA inhibited HS biosynthesis in G. lucidum by binding to the promoter of cbs, a key gene for HS biosynthesis, at "CTGGGG". In our study, we reported for the first time that HS increased the cellulose utilization in G. lucidum, and analyzed the mechanism of HS biosynthesis induced by cellulose. This study not only enriches the understanding of the microbial cellulose utilization mechanism but also provides a reference for the analysis of the physiological function of HS signals.
纤维素是地球上一种重要的丰富可再生资源，微生物利用纤维素的机制引起了广泛关注。最近，发现一些信号分子可以调节纤维素的利用，而对潜在信号的研究最近引起了广泛关注。在本文中，我们发现灵芝在纤维素培养条件下的硫化氢（HS）浓度比葡萄糖培养条件下增加了约 2.3 倍。进一步的证据表明，通过增加 HS 浓度，灵芝的纤维素酶活性提高了 18.2-27.6%。然后，我们观察到碳阻遏物 CreA 通过结合到 HS 生物合成关键基因 cbs 的启动子“CTGGGG”上，抑制灵芝中 HS 的生物合成。在我们的研究中，我们首次报道 HS 增加了灵芝对纤维素的利用，并分析了纤维素诱导 HS 生物合成的机制。这项研究不仅丰富了对微生物纤维素利用机制的理解，也为 HS 信号的生理功能分析提供了参考。
