Key Laboratory of Modern Agricultural Equipment and Technology of Ministry of Education, School of Agricultural Engineering, Jiangsu University, Zhenjiang, China.
State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang, China.
Plant Signal Behav. 2021 Nov 2;16(11):1950899. doi: 10.1080/15592324.2021.1950899. Epub 2021 Jul 6.
The circadian clock regulates a wide range of physiological processes in plants. Here we showed the circadian variations of the electrical signals in L. and L. in a natural state, which were analyzed using the day-night cycle method. The circadian characteristics of different plant electrical signals were compared by constructing a coupling model for the circadian rhythm of plant electrical signals. The electrical signal sensor had two electrode plates, which were fixed on the two ends of the splint, leaves could then be clamped and measured. The clamping force between the two electrode plates was uniform, which enabled continuous and nondestructive measurements. The results showed that an electric cyclic behavior was observed (circadian cycle) with the circadian variation in the plants within 24 h. Both the resistance (R) and the impedance (Z) increased firstly in the early morning and then decreased subsequently, while the capacitance (C) showed an opposite variation. Under different weather conditions, plant electrical signals showed periodic changes when the temperature and light intensity in the environment slightly changed within the physiological tolerance of plant. This indicated that the circadian clock of plant electrical signals could be maintained endogenously. The variation curves of plant electrical signals as time increased were fitted using the sine equation. The characteristic parameters of circadian rhythm of plant electrical signals were obtained. We found that although all plant electrical signals exhibited electric cyclic behavior, but the characteristics of circadian rhythms of electrical signals were different. This study provided a scientific basic for precisely monitoring plant electrical signals, and a reference for revealing circadian rhythms of plant electrical signals and their occurrence rules.
生物钟调节着植物中广泛的生理过程。在这里，我们展示了 L. 和 L. 在自然状态下电信号的昼夜节律变化，这些变化是通过昼夜周期法进行分析的。通过构建植物电信号的生物钟耦合模型，比较了不同植物电信号的昼夜特征。电信号传感器有两个电极板，固定在夹板的两端，然后可以夹住和测量叶片。两个电极板之间的夹持力均匀，从而实现了连续和非破坏性的测量。结果表明，在 24 小时内，植物中观察到了电循环行为（昼夜节律），具有昼夜变化。电阻 (R) 和阻抗 (Z) 首先在清晨增加，然后随后降低，而电容 (C) 则表现出相反的变化。在不同的天气条件下，当环境中的温度和光照强度在植物的生理耐受范围内略有变化时，植物电信号表现出周期性的变化。这表明植物电信号的生物钟可以内源性维持。通过正弦方程拟合植物电信号随时间增加的变化曲线，得到了植物电信号生物钟的特征参数。我们发现，虽然所有植物电信号都表现出电循环行为，但电信号的昼夜节律特征不同。这项研究为精确监测植物电信号提供了科学基础，为揭示植物电信号的昼夜节律及其发生规律提供了参考。
