Division of Dermatology and Venereology, Department of Clinical Sciences, Lund University, Lund, Sweden.
LEO Foundation Center for Cutaneous Drug Delivery, Department of Pharmacy, University of Copenhagen, Copenhagen, Denmark.
Elife. 2021 Jul 6;10:e66876. doi: 10.7554/eLife.66876.
The normal wound healing process is characterised by proteolytic events, whereas infection results in dysfunctional activations by endogenous and bacterial proteases. Peptides, downstream reporters of these proteolytic actions, could therefore serve as a promising tool for diagnosis of wounds. Using mass-spectrometry analyses, we here for the first time characterise the peptidome of human wound fluids. Sterile post-surgical wound fluids were found to contain a high degree of peptides in comparison to human plasma. Analyses of the peptidome from uninfected healing wounds and -infected wounds identify unique peptide patterns of various proteins, including coagulation and complement factors, proteases, and antiproteinases. Together, the work defines a workflow for analysis of peptides derived from wound fluids and demonstrates a proof-of-concept that such fluids can be used for analysis of qualitative differences of peptide patterns from larger patient cohorts, providing potential biomarkers for wound healing and infection.
正常的伤口愈合过程以蛋白水解事件为特征，而感染会导致内源性和细菌蛋白酶的功能失调激活。因此，这些蛋白水解作用的下游报告肽可以作为伤口诊断的有前途的工具。在这里，我们首次使用质谱分析方法对人伤口液的肽组进行了描述。与人类血浆相比，无菌手术后的伤口液中含有高度的肽。对未感染愈合伤口和感染伤口的肽组分析确定了各种蛋白质的独特肽模式，包括凝血和补体因子、蛋白酶和抗蛋白酶。总之，这项工作定义了一个用于分析伤口液衍生肽的工作流程，并证明了这样的液体可以用于分析来自更大患者群体的肽模式的定性差异，为伤口愈合和感染提供潜在的生物标志物。
