Rao G H
Department of Laboratory Medicine and Pathology, University of Minnesota Health Sciences Center, University Hospitals and Clinics, Minneapolis 55455.
Prostaglandins Leukot Med. 1987 Dec;30(2-3):133-45. doi: 10.1016/0262-1746(87)90143-0.
The influence of in vitro treatment of platelets with antiplatelet drugs on the interaction of these cells with the subendothelium was studied using citrated human blood obtained from normal control donors. Reconstituted blood following drug treatment was circulated through a special chamber which housed everted segments of de-endothelialized rabbit aorta. The wall shear rate used in these studies was 800 sec-1. Surface coverage of platelets on the subendothelium were morphometrically evaluated. Aspirin, Ibuprofen, Prostaglandin E1 and 13-Azaprostanoic acid significantly reduced platelet thrombi on exposed subendothelium. The calcium antagonists, Quin 2 and Diltiazem, exerted similar inhibitory effects, whereas Verapamil was a poor inhibitor. Aspirin treatment significantly enhanced platelet adhesion to the exposed vascular surface. Salicylate and Salicylamide did not enhance platelet adherence. Only Aspirin enhanced the formation of lipoxygenase metabolites of radiolabeled arachidonate. Results suggest that drugs which inhibit platelet aggregation and secretion of granule contents reduce formation of platelet thrombi. However, these drugs may or may not have a similar influence on platelet interaction with the subendothelium leading to spreading, adherence or formation of aggregates.
使用从正常对照供体获得的枸橼酸化人血，研究了用抗血小板药物对血小板进行体外处理对这些细胞与内皮下层相互作用的影响。药物处理后的重组血液通过一个特殊的腔室循环，该腔室容纳去内皮化兔主动脉的外翻段。这些研究中使用的壁剪切速率为800秒-1。通过形态计量学评估内皮下层上血小板的表面覆盖率。阿司匹林、布洛芬、前列腺素E1和13-氮杂前列腺酸显著减少暴露内皮下层上的血小板血栓。钙拮抗剂喹诺酮2和地尔硫卓发挥了类似的抑制作用，而维拉帕米是一种较差的抑制剂。阿司匹林处理显著增强血小板对暴露血管表面的粘附。水杨酸盐和水杨酰胺不增强血小板粘附。只有阿司匹林增强了放射性标记花生四烯酸的脂氧合酶代谢产物的形成。结果表明，抑制血小板聚集和颗粒内容物分泌的药物会减少血小板血栓的形成。然而，这些药物可能对也可能不对血小板与内皮下层的相互作用产生类似影响，这种相互作用会导致血小板铺展、粘附或聚集体形成。
