Chao T J, Chang C P, Chang M C, Liu H C, Wang J, Yin S J
Department of Pathology, Tri-Service General Hospital, Taipei, Taiwan, Republic of China.
Proc Natl Sci Counc Repub China B. 1987 Jul;11(3):260-5.
Alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) isoenzyme phenotypes were determined in 71 autopsy liver specimens by agarose isoelectric focusing and starch gel electrophoresis, respectively. Ninety percent of the livers examined exhibited a pH-optimum for ethanol oxidation at pH 8.5, instead of 10.5 which is usual among Caucasians. The homozygous ADH2 2-2 phenotype was found to be 59%, and the heterozygous ADH2 2-1 phenotype, 31%. The remaining 10% of the livers, exhibiting a pH-optimum at 10.5, were homozygous ADH2 1-1 phenotype. Accordingly, the gene frequencies of the alleles ADH2(2) and ADH2(1) were calculated to be 0.75 and 0.25, respectively. Fifty-eight percent of the 71 livers examined possessed both the activities of ALDH I and ALDH II isoenzymes, whereas 42% lacked the ALDH I activity. The implications of these distributions of liver ADH and ALDH isoenzyme patterns among Orientals for ethanol elimination, alcohol sensitivity and alcoholism are discussed.
分别采用琼脂糖等电聚焦法和淀粉凝胶电泳法，对71份尸检肝脏标本的乙醇脱氢酶（ADH）和乙醛脱氢酶（ALDH）同工酶表型进行了测定。90%的受检肝脏乙醇氧化的最适pH值为8.5，而非白种人中常见的10.5。发现纯合子ADH2 2-2表型占59%，杂合子ADH2 2-1表型占31%。其余10%的肝脏最适pH值为10.5，为纯合子ADH2 1-1表型。据此，计算出等位基因ADH2(2)和ADH2(1)的基因频率分别为0.75和0.25。71份受检肝脏中，58%同时具有ALDH I和ALDH II同工酶活性，而42%缺乏ALDH I活性。文中讨论了东方人肝脏ADH和ALDH同工酶模式的这些分布情况对乙醇消除、酒精敏感性和酒精中毒的影响。
