School of Chinese Materia Medica, Guangdong Pharmaceutical University, Guangzhou, China.
School of Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan, China.
Biosci Biotechnol Biochem. 2021 Aug 25;85(9):2054-2064. doi: 10.1093/bbb/zbab126.
The present study was conducted to evaluate the effect of Forsythiae Fructus aqueous extract (FAE) against cisplatin-induced emesis and to explore the antiemetic mechanism of FAE by focusing on NLRP3 inflammasome activation in a rat pica model. Our results showed that FAE significantly ameliorated cisplatin-induced acute and delayed pica in rats. Moreover, FAE improved the gastrointestinal histopathological injury and reduced the levels of serum ROS, IL-1β, and IL-18 in cisplatin-treated rats. In addition, the expressions of NLRP3, ASC, caspase-1, and IL-1β and the colocalization of the NLRP3 with ASC or caspase-1 in rat gastric antrum and ileum were also suppressed by FAE. Taken together, our findings indicate that FAE has a therapeutic effect against CINV, which may be related to its inhibition of the activation of NLRP3 inflammasome.
本研究旨在评估连翘果水提物（FAE）对顺铂诱导的呕吐的作用，并通过关注 NLRP3 炎性体激活在大鼠异食癖模型中探索 FAE 的止吐机制。我们的结果表明，FAE 可显著改善顺铂诱导的大鼠急性和迟发性异食癖。此外，FAE 改善了顺铂处理大鼠的胃肠道组织病理学损伤，并降低了血清 ROS、IL-1β 和 IL-18 的水平。此外，FAE 还抑制了 NLRP3、ASC、半胱天冬酶-1 和 IL-1β 在大鼠胃窦和回肠中的表达，以及 NLRP3 与 ASC 或半胱天冬酶-1 的共定位。综上所述，我们的研究结果表明，FAE 对 CINV 具有治疗作用，这可能与其抑制 NLRP3 炎性体的激活有关。
