Scherwitz C, Rassner G
Geburtshilfe Frauenheilkd. 1978 Jan;38(1):53-7.
Experience with the germ tube test for the diagnosis of candida mycosis in gynaecology and dermatology is reported. Candida albicans forms germ tubes in human serum and other culture media at 37 degrees C within 2 to 3 hours. If the initial culture grows yeast, the germ tube test permits the exact identification of the yeast as candida albicans within three to five hours. The specificity of the test is approximately 99%. Of 3270 samples 934 grew yeast and 742 were candida albicans by the germ tube test. 6 samples with a negative germ tube test were identified as candida albicans by the Chlamydospore test. 125 samples showed a candida species, 45 samples showed a torulopsis species and 16 tests identified a trichosporon species. This test is valuable in the routine office practice of gynaecology because of its simplicity and speed.
本文报告了芽管试验在妇科和皮肤科念珠菌病诊断中的应用经验。白色念珠菌在人血清和其他培养基中于37℃下2至3小时内形成芽管。如果初始培养物生长出酵母,芽管试验可在三至五小时内准确鉴定该酵母为白色念珠菌。该试验的特异性约为99%。在3270份样本中,934份生长出酵母,通过芽管试验鉴定出742份为白色念珠菌。6份芽管试验阴性的样本通过厚壁孢子试验鉴定为白色念珠菌。125份样本显示为念珠菌属,45份样本显示为球拟酵母菌属,16份试验鉴定为毛孢子菌属。由于其简单性和快速性,该试验在妇科日常门诊实践中具有重要价值。