Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, United States of America.
Department of Pathology and Cell Biology, USF Health Heart Institute, University of South Florida, Tampa, FL, United States of America.
PLoS One. 2021 Jul 7;16(7):e0254024. doi: 10.1371/journal.pone.0254024. eCollection 2021.
During embryonic development, cells differentiate into a variety of distinct cell types and subtypes with diverse transcriptional profiles. To date, transcriptomic signatures of different cell lineages that arise during development have been only partially characterized. Here we used single-cell RNA-seq to perform transcriptomic analysis of over 20,000 cells disaggregated from the trunk region of zebrafish embryos at the 30 hpf stage. Transcriptional signatures of 27 different cell types and subtypes were identified and annotated during this analysis. This dataset will be a useful resource for many researchers in the fields of developmental and cellular biology and facilitate the understanding of molecular mechanisms that regulate cell lineage choices during development.
在胚胎发育过程中，细胞分化为具有不同转录谱的多种不同细胞类型和亚型。迄今为止，发育过程中出现的不同细胞谱系的转录组特征仅部分得到了描述。在这里，我们使用单细胞 RNA-seq 对来自斑马鱼胚胎 30 hpf 阶段躯干区域的超过 20,000 个细胞进行了转录组分析。在该分析过程中鉴定并注释了 27 种不同细胞类型和亚型的转录特征。这个数据集将成为发育和细胞生物学领域许多研究人员的有用资源，并有助于理解调控发育过程中细胞谱系选择的分子机制。
