Connell Mackenzie L, Meyer Danielle N, Haimbaugh Alex, Baker Tracie R
University of Florida, Department of Environmental & Global Health, 2173 Mowry Rd, Gainesville, FL 32611, USA.
Curr Opin Toxicol. 2024 Jun;38. doi: 10.1016/j.cotox.2024.100463. Epub 2024 Jan 26.
The utilization of transcriptomic studies identifying profiles of gene expression, especially in toxicogenomics, has catapulted next-generation sequencing to the forefront of reproductive toxicology. An innovative yet underutilized RNA sequencing technique emerging into this field is single-cell RNA sequencing (scRNA-seq), which provides sequencing at the individual cellular level of gonad tissue. ScRNA-seq provides a novel and unique perspective for identifying distinct cellular profiles, including identification of rare cell subtypes. The specificity of scRNA-seq is a powerful tool for reproductive toxicity research, especially for translational animal models including zebrafish. Studies to date not only have focused on 'tissue atlassing' or characterizing what cell types make up different tissues but have also begun to include toxicant exposure as a factor that this review aims to explore. Future scRNA-seq studies will contribute to understanding exposure-induced outcomes; however, the trade-offs with traditional methods need to be considered.
转录组学研究在识别基因表达谱方面的应用，尤其是在毒理基因组学中的应用，已将下一代测序技术推到了生殖毒理学的前沿。一种新兴的、尚未得到充分利用的RNA测序技术——单细胞RNA测序（scRNA-seq）正进入这一领域，它能在性腺组织的单个细胞水平上进行测序。scRNA-seq为识别不同的细胞谱提供了一个新颖而独特的视角，包括识别罕见的细胞亚型。scRNA-seq的特异性是生殖毒性研究的有力工具，特别是对于包括斑马鱼在内的转化动物模型。迄今为止的研究不仅集中在“组织图谱绘制”或描述构成不同组织的细胞类型，还开始将毒物暴露作为一个因素纳入研究，而这正是本综述旨在探讨的内容。未来的scRNA-seq研究将有助于理解暴露诱导的结果；然而，需要考虑与传统方法的权衡。
