分枝杆菌和分枝杆菌感染免疫细胞的流式细胞术分析。
Flow Cytometry Analysis of Mycobacteria and Mycobacteria-Infected Immune Cells.
机构信息
Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA, USA.
出版信息
Methods Mol Biol. 2021;2314:261-271. doi: 10.1007/978-1-0716-1460-0_11.
Flow cytometry enables the measurement of tens of features on individual cells from complex mixtures. Flow cytometry enables high-throughput quantification of cell size, gene and protein expression. In the case of studies of host-pathogen interactions, this tool provides a facile way of identifying cells that have been successfully infected by a pathogen. Several recent technological advances have greatly improved throughput and the number of features that can be simultaneously monitored by this technique. Here, we describe common workflows to study Mycobacterium tuberculosis heterogeneity and host-M. tuberculosis interactions using flow cytometry and related technologies.
流式细胞术能够在复杂混合物中对单个细胞进行数十种特征的测量。流式细胞术能够高通量地定量细胞大小、基因和蛋白质表达。在研究宿主-病原体相互作用的情况下，该工具提供了一种简单的方法来识别已被病原体成功感染的细胞。最近的几项技术进步极大地提高了该技术的通量和可以同时监测的特征数量。在这里，我们描述了使用流式细胞术和相关技术研究结核分枝杆菌异质性和宿主-结核分枝杆菌相互作用的常见工作流程。
Zotero 插件