Department of Chemical and Biological Engineering, Princeton University, Princeton, New Jersey 08544, United States.
Langmuir. 2021 Jul 20;37(28):8517-8524. doi: 10.1021/acs.langmuir.1c00990. Epub 2021 Jul 8.
Chitosan-coated nanoparticles are a promising class of drug delivery vehicles that have been studied as tools for improving the gastrointestinal delivery of therapeutics. Here we present an analysis of chitosan-coated nanoparticles with an emphasis on characterizing the chitosan polymer properties. Cationic nanoparticles are produced by adsorbing a layer of chitosan HCl on an anionic (-40 mV ζ-potential) polyacrylic acid (PAA) coated primary nanoparticle. Commercially available chitosan (90% deacetylated) must be processed into a nearly completely deacetylated HCl salt form (99% deacetylation); otherwise, primary nanoparticle aggregation occurs. Deacetylated chitosan HCl produces stable, cationic (+35 mV ζ-potential) nanoparticles within 10% of the original anionic particle hydrodynamic diameter at a 1:2 molar ratio of chitosan glucosamine HCl monomers to PAA acrylic acid monomers.
壳聚糖包覆的纳米颗粒是一类很有前途的药物输送载体，已被研究用于改善治疗药物在胃肠道中的传递。在这里，我们介绍了壳聚糖包覆的纳米颗粒的分析，重点是对壳聚糖聚合物特性进行了表征。阳离子纳米颗粒是通过在带负电荷（-40 mV ζ-电位）的聚丙烯酸（PAA）涂层初级纳米颗粒上吸附一层壳聚糖盐酸盐来制备的。商业上可用的壳聚糖（90%脱乙酰度）必须处理成几乎完全脱乙酰的盐酸盐形式（99%脱乙酰度）；否则，初级纳米颗粒会发生聚集。在壳聚糖氨基葡萄糖盐酸盐与 PAA 丙烯酸单体的摩尔比为 1:2 时，脱乙酰壳聚糖盐酸盐在 10%的原始阴离子颗粒水动力直径内产生稳定的阳离子（+35 mV ζ-电位）纳米颗粒。
