Lutz H U, Stammler P, Furter C, Fasler S
Laboratorium für Biochemie, ETH Zürich.
Schweiz Med Wochenschr. 1987 Nov 14;117(46):1821-4.
Naturally occurring antibodies against the anion exchange protein of red cells (band 3 protein) can elicit in whole serum a strong C3b deposition to red cells under conditions which favor alternative complement pathway activation. Such a mode of opsonization calls for generation of an alternative C3 convertase nucleated by C3b covalently bound to anti-band 3. Senescent, but not young, red cells should also carry "C3b-anti-band 3" complexes, if clearance of in vivo aged red cells occurred by the same mechanism. We succeeded in isolating covalently linked complexes of C3b and IgG primarily from membranes of senescent red cells.
针对红细胞阴离子交换蛋白（带3蛋白）的天然抗体，在有利于替代补体途径激活的条件下，可在全血清中引发强烈的C3b沉积于红细胞。这种调理作用模式需要由与抗带3共价结合的C3b形成核的替代C3转化酶的产生。如果体内衰老红细胞的清除是通过相同机制进行的，那么衰老而非年轻的红细胞也应携带“C3b - 抗带3”复合物。我们成功地主要从衰老红细胞膜中分离出了C3b和IgG的共价连接复合物。
