Berczi I, Sehon A H
Immunol Commun. 1977;6(6):617-32. doi: 10.3109/08820137709093471.
The effect of cytotoxic lymphoid cells emerging in primary cultures of an antigenic sarcoma of strain 13 guinea pigs was investigated on tumor growth in vivo. The growth of lethal tumor inocula was inhibited or the tumor cells were completely rejected in normal syngeneic recipients treated s.c. with low doses (5 X 10(5)) of effector cells which were mixed with the tumor cells. A higher dose (2.5 X 10(6)) of killer cells did not affect significantly tumor growth in normal recipients. Significant tumor inhibition was observed in X-irradiated recipients given high doses (2.2 X 10(6)) of effector cells locally although rejection did not occur with high frequency. Local treatment of X-irradiated recipients with low doses (5 X 10(5)) of effector cells did not influence tumor growth. Systemic treatment of normal or X-irradiated recipients with effector cells had little effect at any dose, although tumor inocula were occasionally rejected. Sonication of the cytotoxic effector cells prior to administration inhibited their tumor suppressing effect. Animals that rejected the first tumor inoculum were immune to a second lethal dose of sarcoma cells. It is indicated by the results that tumor rejection in vivo is not simply a matter of killer-target cell interaction, but rather a complex and poorly understood phenomenon.
研究了13号品系豚鼠抗原性肉瘤原代培养物中出现的细胞毒性淋巴细胞对体内肿瘤生长的影响。在皮下注射低剂量（5×10⁵）与肿瘤细胞混合的效应细胞的同基因正常受体中，致死性肿瘤接种物的生长受到抑制或肿瘤细胞被完全排斥。较高剂量（2.5×10⁶）的杀伤细胞对正常受体中的肿瘤生长没有显著影响。在局部给予高剂量（2.2×10⁶）效应细胞的受X射线照射的受体中观察到显著的肿瘤抑制，尽管排斥反应并不频繁发生。用低剂量（5×10⁵）效应细胞对受X射线照射的受体进行局部治疗不影响肿瘤生长。用效应细胞对正常或受X射线照射的受体进行全身治疗，无论剂量如何，效果都很小，尽管肿瘤接种物偶尔会被排斥。在给药前对细胞毒性效应细胞进行超声处理会抑制其肿瘤抑制作用。排斥第一个肿瘤接种物的动物对第二次致死剂量的肉瘤细胞具有免疫力。结果表明，体内肿瘤排斥不仅仅是杀伤细胞与靶细胞相互作用的问题，而是一个复杂且了解甚少的现象。
