Lv Ya-Xin, Pan Hao-Ran, Song Xin-Ying, Chang Qing-Qi, Zhang Dan-Dan
Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Shanghai Zhangjiang Group Middle School, Shanghai 201203, China.
Evid Based Complement Alternat Med. 2021 Jun 14;2021:6666499. doi: 10.1155/2021/6666499. eCollection 2021.
(HD) plus (SB) have been widely used in antitumor clinical prescribes as one of herb pairs in China. We investigated the effect of aqueous extract from plus at the equal weight ratio (HDSB11) in inhibiting the growth of murine non-small-cell lung cancer cell (NSCLC) line LLC and in this study. Compared with other aqueous extracts, HDSB11 showed the lowest IC in inhibiting cell proliferation at 0.43 mg/ml. Besides, HDSB11 effectively suppressed colony formation and induced cell apoptosis. The further assessment of HDSB11 on the murine Lewis-lung-carcinoma-bearing mouse model showed it significantly inhibited tumors' bioluminescence at the dose of 30 g crude drug/kg. Mechanistically, HDSB11 attenuated the expressions of NLRP3, procaspase-1, caspase-1, PRAP, Bcl-2, and cyclin D1 and downregulated the phosphorylation levels of NF-B, ERK, JNK, and p38 MAPK. In conclusion, HDSB11 could alleviate cell proliferation and colony formation and induce apoptosis and tumor growth , partly via NF-B and MAPK signaling pathways to suppress NLRP3 expression.
（HD）加（SB）作为中药药对之一已在中国抗肿瘤临床处方中广泛应用。本研究中，我们考察了等重量比的（HD）加（SB）水提取物（HDSB11）对小鼠非小细胞肺癌细胞（NSCLC）系LLC生长的抑制作用。与其他水提取物相比，HDSB11在抑制细胞增殖方面表现出最低的半数抑制浓度（IC），为0.43mg/ml。此外，HDSB11有效抑制集落形成并诱导细胞凋亡。对荷小鼠Lewis肺癌模型进一步评估显示，HDSB11在30g生药/kg剂量下显著抑制肿瘤生物发光。机制上，HDSB11减弱了NLRP3、procaspase-1、caspase-1、PRAP、Bcl-2和细胞周期蛋白D1的表达，并下调了NF-κB、ERK、JNK和p38 MAPK的磷酸化水平。总之，HDSB11可减轻细胞增殖和集落形成，诱导细胞凋亡并抑制肿瘤生长，部分是通过NF-κB和MAPK信号通路抑制NLRP3表达实现的。
