Wang Baikui, Ye Xiaolin, Zhou Yuanhao, Zhao Pengwei, Mao Yulong
Key Laboratory of Animal Molecular Nutrition of Education of Ministry, National Engineering Laboratory of Biological Feed Safety and Pollution Prevention and Control, Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, Institute of Animal Nutrition and Feed Sciences, College of Animal Sciences, Zhejiang University, Hangzhou, China.
Medical Faculty, Heinrich-Heine University Düsseldorf, Düsseldorf, Germany.
Front Vet Sci. 2021 Jun 22;8:648698. doi: 10.3389/fvets.2021.648698. eCollection 2021.
are one of the most important foodborne pathogens, which threaten the health of humans and animals severely. Glycyrrhizin (GL) has been proven to exhibit anti-inflammatory and tissue-protective properties. Here, we investigated the effects of GL on tissue injury, inflammatory response, and intestinal dysbiosis in Typhimurium-infected mice. Results showed that GL or gentamicin (GM) significantly ( < 0.05) alleviated ST-induced splenomegaly indicated by the decreased spleen index, injury of liver and jejunum indicated by the decreased hepatocytic apoptosis, and the increased jejunal villous height. GL significantly ( < 0.05) increased secretion of inflammatory cytokines (IFN-γ, IL-12p70, IL-6, and IL-10) in spleen and IL-12p40 mRNA expression in liver. Meanwhile, GL or GM pre-infection treatments significantly ( < 0.05) decreased ST-induced pro-inflammatory cytokine (IFN-γ, TNF-α, and IL-6) expression in both spleen and liver and increased ( < 0.05) anti-inflammatory cytokine IL-10 secretion in spleen. Furthermore, GL or GM pre-infection treatment also regulates the diversities and compositions of intestinal microbiota and decreased the negative connection among the intestinal microbes in ST-infected mice. The above findings indicate that GL alleviates ST-induced splenomegaly, hepatocytic apoptosis, injury of jejunum and liver, inflammatory response of liver and spleen, and intestinal dysbacteriosis in mice.
是最重要的食源性病原体之一，严重威胁人类和动物的健康。甘草酸（GL）已被证明具有抗炎和组织保护特性。在此，我们研究了GL对鼠伤寒沙门氏菌感染小鼠的组织损伤、炎症反应和肠道菌群失调的影响。结果表明，GL或庆大霉素（GM）显著（<0.05）减轻了ST诱导的脾肿大，表现为脾脏指数降低；减轻了肝脏和空肠损伤，表现为肝细胞凋亡减少以及空肠绒毛高度增加。GL显著（<0.05）增加了脾脏中炎性细胞因子（IFN-γ、IL-12p70、IL-6和IL-10）的分泌以及肝脏中IL-12p40 mRNA的表达。同时，GL或GM预感染处理显著（<0.05）降低了ST诱导的脾脏和肝脏中促炎细胞因子（IFN-γ、TNF-α和IL-6）的表达，并增加了（<0.05）脾脏中抗炎细胞因子IL-10的分泌。此外，GL或GM预感染处理还调节了肠道微生物群的多样性和组成，并减少了ST感染小鼠肠道微生物之间的负相关联系。上述发现表明，GL减轻了ST诱导的小鼠脾肿大、肝细胞凋亡、空肠和肝脏损伤、肝脏和脾脏的炎症反应以及肠道菌群失调。
