Department of Radiology, The Second Hospital, Dalian Medical University, Dalian, China.
Department of Pathology, The Second Hospital, Dalian Medical University, Dalian, China.
J Magn Reson Imaging. 2022 Jan;55(1):225-233. doi: 10.1002/jmri.27828. Epub 2021 Jul 8.
High cellularity and abnormal interstitial structures are some of the unfavorable factors that affect the treatment outcomes and survival of rhabdomyosarcoma (RMS) patients.
To explore the correlation between diffusion-weighted imaging (DWI) and intravoxel incoherent motion (IVIM) with quantitative histopathologic features in a murine model of RMS.
Prospective.
Murine model of RMS (31 female BALB/c nude mice).
FIELD STRENGTH/SEQUENCE: 3.0 T; fast spin-echo (FSE) T1-weighted imaging, fast relaxation fast spin-echo (FRFSE) T2-weighted imaging, DWI PROPELLER FSE imaging sequence, and IVIM echo planar imaging sequence; 10 different b-values (0, 50, 100, 150, 200, 400, 600, 800, 1000, and 1200 s/mm ).
Magnetic resonance imaging (MRI) was performed after 30-45 days of implantation. The following MRI parameters were calculated: apparent diffusion coefficient (ADC), pure diffusion coefficient (D), pseudo-diffusion coefficient (D*), and perfusion fraction (f). Histopathologic features, which contained nuclear, cytoplasmic, and stromal fractions, and the nuclear-to-cytoplasmic ratio within the tumor were measured using image-based segmentation.
Pearson's correlation, multiple linear regression analysis, and receiver operating characteristic curve analysis were performed. A P < 0.05 was considered statistically significant.
The ADC value showed moderate negative correlation with nuclear fraction (r = -0.540), and moderate positive correlation with stroma fraction (r = 0.474). The D value showed moderate negative correlation with nuclear fraction (r = -0.491), and moderate positive correlation with stroma fraction (r = 0.421). The f value showed a moderate negative correlation with stroma fraction (r = -0.423). The D value showed the best diagnostic ability. The optimal cut-off D value of 0.460 was associated with 77.8% sensitivity and 68.2% specificity (area under the curve, 0.747).
The ADC, D, and f values obtained from DWI and IVIM images showed moderate correlation with the quantitative histopathologic features in a murine model of RMS.
1 TECHNICAL EFFICACY STAGE: 3.
高细胞密度和异常的间质结构是影响横纹肌肉瘤（RMS）患者治疗效果和生存的不利因素之一。
探讨扩散加权成像（DWI）和体素内不相干运动（IVIM）与 RMS 小鼠模型的定量组织病理学特征之间的相关性。
前瞻性。
RMS 小鼠模型（31 只雌性 BALB/c 裸鼠）。
磁场强度/序列：3.0T；快速自旋回波（FSE）T1 加权成像、快速弛豫快速自旋回波（FRFSE）T2 加权成像、DWI 推进式 FSE 成像序列和 IVIM 平面回波成像序列；10 个不同的 b 值（0、50、100、150、200、400、600、800、1000 和 1200 s/mm）。
植入后 30-45 天进行 MRI 检查。计算以下 MRI 参数：表观扩散系数（ADC）、纯扩散系数（D）、假性扩散系数（D*）和灌注分数（f）。使用基于图像的分割方法测量肿瘤内的核、细胞质和基质分数以及核-质比。
进行 Pearson 相关性、多元线性回归分析和受试者工作特征曲线分析。P<0.05 被认为具有统计学意义。
ADC 值与核分数呈中度负相关（r=-0.540），与基质分数呈中度正相关（r=0.474）。D 值与核分数呈中度负相关（r=-0.491），与基质分数呈中度正相关（r=0.421）。f 值与基质分数呈中度负相关（r=-0.423）。D 值具有最佳的诊断能力。最佳截断值 D=0.460 时，灵敏度为 77.8%，特异性为 68.2%（曲线下面积为 0.747）。
DWI 和 IVIM 图像的 ADC、D 和 f 值与 RMS 小鼠模型的定量组织病理学特征具有中度相关性。
1 技术功效分期：3。
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