Department of Radiology, The Second Hospital, Dalian Medical University, Dalian, Liaoning, People's Republic of China.
Department of Radiology, Suzhou Hospital of Anhui Medical University, Suzhou, Anhui, People's Republic of China.
Magn Reson Med. 2022 Aug;88(2):871-879. doi: 10.1002/mrm.29250. Epub 2022 Apr 4.
To investigate the correlation between DWI, intravoxel incoherent motion (IVIM), and hypoxia-inducible factor 1-alpha (HIF-1α) expression in a nude mouse model of rhabdomyosarcoma based on imaging and pathological comparisons.
Human rhabdomyosarcoma-derived (RD) cells were inoculated into the right thigh muscle of 20 BALB/c female nude mice. Mice were imaged using 3.0 Tesla MRI system. T -weighted imaging, T -weighted imaging, DWI, and IVIM images were obtained. ADW4.7 (GE Healthcare, ChicagoAQ34, IL, USA) was used for image processing of ADC, D , D , and f values. All parameter values were independently analyzed by 2 observers. Immunohistochemistry of HIF-1α was performed. We used a specific image-pathology comparison method to ensure correct overlap between the image plane and the pathological section. Mann-Whitney U test or independent sample t test, Pearson or Spearman correlation test, the intragroup correlation coefficient, Kolmogorov-Smirnov test, and receiver operating characteristic curve were used. The correlation between DWI and intravoxel incoherent motion parameter values and HIF-1α expression was determined.
There were 10 mice in the low-expression group and 7 in the high-expression group. The ADC and D values were negatively correlated with HIF-1α with correlation coefficients of -0.491 and - 0.702 (P = 0.045 and 0.002). The f value positively correlated with HIF-1α expression (r = 0.485, P = 0.048). ADC, D , and f were significantly different between the high-HIF-1α expression tumors and the low-HIF-1α expression tumors. ADC showed the best predictive performance among all parameters (area under the curve = 0.652, sensitivity = 83.3%, specificity = 63.6%).
The parameter values of DWI and intravoxel incoherent motion can be used to evaluate the expression of HIF-1α in rhabdomyosarcoma. ADC, D , and f value showed correlation with the expression of HIF-1α.
通过影像学与病理学对照，研究裸鼠横纹肌肉瘤模型中弥散加权成像（DWI）、体素内不相干运动（IVIM）与缺氧诱导因子 1-α（HIF-1α）表达的相关性。
将人横纹肌肉瘤源性（RD）细胞接种于 20 只 BALB/c 雌性裸鼠右侧大腿肌肉中。采用 3.0 Tesla MRI 系统对小鼠进行成像。获取 T1 加权成像、T2 加权成像、DWI 和 IVIM 图像。使用 ADW4.7（GE Healthcare，Chicago，IL，USA）对 ADC、D、D*和 f 值进行图像处理。两位观察者分别对所有参数值进行独立分析。行 HIF-1α 免疫组化染色。采用特定的图像-病理学对比方法，确保图像平面与病理切片正确重叠。采用 Mann-Whitney U 检验或独立样本 t 检验、Pearson 或 Spearman 相关检验、组内相关系数、Kolmogorov-Smirnov 检验和受试者工作特征曲线，确定 DWI 和体素内不相干运动参数值与 HIF-1α 表达的相关性。
低表达组有 10 只小鼠，高表达组有 7 只小鼠。ADC 和 D 值与 HIF-1α 呈负相关，相关系数分别为-0.491 和-0.702（P=0.045 和 0.002）。f 值与 HIF-1α 表达呈正相关（r=0.485，P=0.048）。高 HIF-1α 表达肿瘤与低 HIF-1α 表达肿瘤之间的 ADC、D*和 f 值存在显著差异。在所有参数中，ADC 具有最佳的预测性能（曲线下面积=0.652，灵敏度=83.3%，特异性=63.6%）。
DWI 和体素内不相干运动参数值可用于评估横纹肌肉瘤中 HIF-1α 的表达。ADC、D*和 f 值与 HIF-1α 的表达呈相关性。
