Arone Coline, Dibsy Rayane, Inamdar Kaushik, Lyonnais Sébastien, Arhel Nathalie J, Favard Cyril, Muriaux Delphine
IRIM, Université de Montpellier, UMR 9004 CNRS, 1919, route de Mende, 34293 Montpellier.
Cemipai, Université de Montpellier, UAR 3725 CNRS, 1919, route de Mende, 34293 Montpellier.
Virologie (Montrouge). 2021 Jun 1;25(3):153-167. doi: 10.1684/vir.2021.0907.
The recent revolution in optical fluorescence microscopy, supported by the optimization of both spatial resolution and acquisition speed, led to the ability to visualize nano-scaled objects. Currently, the use of a new generation of super-resolution fluorescence microscopes coupled to improved fluorescent probes gives the possibility to study the replicative cycle of viruses in living cells, at the single-virus and molecule level. In this review, after a brief chronological description of these new approaches, we highlight several examples of super-resolution microscopies that have allowed to revisit our understanding of several human viruses and of host-pathogen interactions.
近期光学荧光显微镜的革命，得益于空间分辨率和采集速度的优化，实现了对纳米级物体的可视化。目前，新一代超分辨率荧光显微镜与改良的荧光探针相结合，使得在单病毒和分子水平研究活细胞中病毒的复制周期成为可能。在本综述中，在按时间顺序简要描述这些新方法之后，我们重点介绍了几个超分辨率显微镜的实例，这些实例使我们对几种人类病毒以及宿主-病原体相互作用的理解得以更新。
