Laboratory of Biochemistry and Immunology (Kim, Go, Lee, Choi) and Laboratory of Veterinary Dermatology and Neurology (Kang), College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, 28644, Republic of Korea; Veterinary Nutrition Laboratory, JEIL Feed Co. Ltd., Daejeon, Chungnam, Republic of Korea (Cho).
Can J Vet Res. 2021 Jul;85(3):177-185.
From 50 to 60% of companion animals in the United States are overweight or obese and this obesity rate is rising. As obesity is associated with a number of health problems, an agent that can help weight loss in pets and assist in clinically managing obesity through veterinary prescription foods and medication would be beneficial. Many studies have shown that celastrol, a phytochemical compound found in extract (COE), has anti-obesity and anti-inflammatory effects, although these effects have not yet been determined in canine or canine-derived cells. The objective of this study was to investigate the effects of celastrol on the adipogenic differentiation and lipolysis of canine adipocytes. Primary preadipocytes were isolated from the gluteal region of a beagle dog and the primary adipocytes were differentiated into mature adipocytes by adipocyte differentiation media containing isobutylmethylxanthine, dexamethasone, and insulin. In a water-soluble tetrazolium (WST) assay, the cell viability of mature adipocytes was decreased after treatment with COE (0, 0.93, 2.32, and 4.64 nM celastrol) in a concentration-dependent manner, although preadipocytes were not affected. Oil Red O (ORO) staining revealed that COE inhibited the differentiation into mature adipocytes and lipid accumulation in adipocytes. In addition, treatment with COE significantly reduced triglyceride content and increased lipolytic activities by 1.5-fold in canine adipocytes. Overall, it was concluded that COE may enhance anti-obesity activity in canine adipocytes by inhibiting lipid accumulation and increasing lipolytic activity.
在美国，50%到 60%的伴侣动物超重或肥胖，而且这个肥胖率还在上升。由于肥胖与许多健康问题有关，如果有一种可以帮助宠物减肥的药物，并且可以通过兽医处方食品和药物来协助临床管理肥胖，那将是有益的。许多研究表明，从雷公藤中提取的植物化学化合物 celastrol 具有抗肥胖和抗炎作用，尽管这些作用尚未在犬或犬源细胞中得到确定。本研究的目的是研究 celastrol 对犬脂肪细胞的脂肪生成分化和脂肪分解的影响。从小猎犬的臀部区域分离原代前体脂肪细胞，并通过含有异丁基甲基黄嘌呤、地塞米松和胰岛素的脂肪细胞分化培养基将原代脂肪细胞分化为成熟脂肪细胞。在水溶性四唑盐（WST）测定中，成熟脂肪细胞的细胞活力在 COE（0、0.93、2.32 和 4.64 nM celastrol）浓度依赖性处理后降低，而前体脂肪细胞不受影响。油红 O（ORO）染色显示 COE 抑制成熟脂肪细胞分化和脂肪细胞中脂质积累。此外，COE 处理可显著降低犬脂肪细胞中的甘油三酯含量，并使脂肪分解活性增加 1.5 倍。总的来说，COE 可能通过抑制脂质积累和增加脂肪分解活性来增强犬脂肪细胞的抗肥胖活性。
