Novotny C P, Fives-Taylor P
J Bacteriol. 1978 Feb;133(2):459-64. doi: 10.1128/jb.133.2.459-464.1978.
The effects of high temperatures (46 to 50 degrees C) on the production of F pili by Escherichia coli were studied by electron microscopy. Attached F pili rapidly disappeared at 48 and 50 degrees C but not at 46 degrees C. Free pili were not denatured at these temperatures. The pili that disappeared from the cells at 50 degrees C did not appear as free pili in the culture supernatant fluid, indicating that the pili had retracted to the cell surface or into the cell. The adsorption of either R17 phage or F pili antibody to the sides of pili prevented retraction. The disappearance of pili was accompanied by a loss in the ability to adsorb R17 phage but not M13 phage, suggesting that the tip of a pilus remains exposed after retraction.
通过电子显微镜研究了高温（46至50摄氏度）对大肠杆菌F菌毛产生的影响。附着的F菌毛在48和50摄氏度时迅速消失，但在46摄氏度时不会消失。游离菌毛在这些温度下不会变性。在50摄氏度时从细胞上消失的菌毛在培养上清液中并未以游离菌毛的形式出现，这表明菌毛已缩回细胞表面或进入细胞内。R17噬菌体或F菌毛抗体吸附到菌毛侧面可阻止菌毛缩回。菌毛的消失伴随着吸附R17噬菌体能力的丧失，但不影响吸附M13噬菌体的能力，这表明菌毛缩回后其尖端仍暴露在外。
