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利用电子光谱成像技术检测接合性F-样菌毛中的磷酸盐

Examination of the phosphate in conjugative F-like pili by use of electron spectroscopic imaging.

作者信息

Frost L S, Bazett-Jones D P

机构信息

Department of Microbiology, University of Alberta, Edmonton, Canada.

出版信息

J Bacteriol. 1991 Dec;173(23):7728-31. doi: 10.1128/jb.173.23.7728-7731.1991.

DOI:10.1128/jb.173.23.7728-7731.1991
PMID:1682305
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC212548/
Abstract

F-like pili specified by conjugative plasmids have been reported to contain phosphate which may be noncovalently incorporated into the pilus. Electron spectroscopic imaging was able to detect phosphate in the filamentous, single-stranded DNA phage f1, used as positive control, but could not detect phosphate in F-like pili. Thus, the phosphate in purified pili which has been reported is probably derived from contaminating cell envelope material.

摘要

据报道,由接合质粒指定的F样菌毛含有磷酸盐,其可能非共价结合到菌毛中。电子光谱成像能够在用作阳性对照的丝状单链DNA噬菌体f1中检测到磷酸盐,但在F样菌毛中无法检测到磷酸盐。因此,已报道的纯化菌毛中的磷酸盐可能来自污染的细胞膜材料。

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J Bacteriol. 1991 Dec;173(23):7728-31. doi: 10.1128/jb.173.23.7728-7731.1991.
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引用本文的文献

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Mol Cell Biochem. 1995 Jul 19;148(2):165-81. doi: 10.1007/BF00928154.
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Synthesis of F pilin.F菌毛蛋白的合成
J Bacteriol. 1993 Mar;175(5):1384-91. doi: 10.1128/jb.175.5.1384-1391.1993.
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Analysis of the sequence and gene products of the transfer region of the F sex factor.F 性因子转移区域的序列及基因产物分析

本文引用的文献

1
COMPLEXES OF F-PILI AND RNA BACTERIOPHAGE.F菌毛与RNA噬菌体复合物
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Nucleotide sequence of bacteriophage f1 DNA.噬菌体f1 DNA的核苷酸序列。
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Nucleotide sequence and genome organisation of filamentous bacteriophages fl and fd.丝状噬菌体fl和fd的核苷酸序列及基因组结构
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Nature of the carbohydrate and phosphate associated with ColB2 and EDP208 pilin.与ColB2和EDP208菌毛相关的碳水化合物和磷酸盐的性质。
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Comparative biochemical studies on F and EDP208 conjugative pili.F和EDP208接合菌毛的比较生化研究。
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Localization, cloning, and sequence determination of the conjugative plasmid ColB2 pilin gene.接合性质粒ColB2菌毛蛋白基因的定位、克隆及序列测定
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10
Stages in phage R17 infection. 3. Energy requirements for the F-pili mediated eclipse of viral infectivity.噬菌体R17感染的阶段。3. F菌毛介导的病毒感染性隐蔽期的能量需求。
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