Department of Microbiology, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Department of Microbiology, Research Center of Reference Health Laboratory, Ministry of Health and Medical Education, Tehran, Iran.
Front Cell Infect Microbiol. 2021 Jun 23;11:560622. doi: 10.3389/fcimb.2021.560622. eCollection 2021.
AmpC β-lactamases hydrolyze all β-lactams except cefepime and carbapenems. The study of AmpC-producing has high priority for the infection control committee. This research is aimed to investigate the resistant urinary AmpC-generating isolates and identify their genetic variety. Some 230 isolates from patients suffering urinary tract infection symptoms were studied in 2017-2018 to assess their susceptibility toward antimicrobial agents. AmpC gene was evaluated by PCR and molecular typing using the 10-loci MLVA method. MLVA images were examined by BioNumerics 6.6 software through the use of the UPGMA algorithms. Thirty-eight AmpC-generating isolates were detected. The most abundant determinant was and , , and had the next ranks, respectively. Six major clusters and a singleton were identified by MLVA. AmpC beta-lactamases in urinary isolates of in the hospital under study and high rate of additional resistance to gentamicin, cotrimoxazole and ciprofloxacin. The most frequent gene determinant of AmpC beta-lactamase was and vary depending on time and geographical location.
AmpCβ-内酰胺酶水解除头孢吡肟和碳青霉烯类以外的所有β-内酰胺类抗生素。产 AmpC 酶的研究对感染控制委员会具有高度优先性。本研究旨在调查耐药性尿源产 AmpC 酶 的分离株及其遗传多样性。对 2017-2018 年间 230 例患有尿路感染症状的患者的分离株进行研究，以评估其对抗菌药物的敏感性。采用 PCR 方法检测 AmpC 基因，并使用 10 个基因座 MLVA 方法进行分子分型。使用 UPGMA 算法通过 BioNumerics 6.6 软件对 MLVA 图谱进行分析。检测到 38 株产 AmpC 酶 。最常见的决定因素是 ，其次是 、 和 。通过 MLVA 鉴定出 6 个主要聚类和一个单峰。研究医院尿标本中产 AmpC 酶的 分离株以及对庆大霉素、复方新诺明和环丙沙星的高附加耐药率。AmpCβ-内酰胺酶的最常见基因决定因素是 ，其取决于时间和地理位置而有所不同。
