AmpR在质粒编码的AmpCβ-内酰胺酶CFE-1高表达中的作用
Role of AmpR in the High Expression of the Plasmid-Encoded AmpC β-Lactamase CFE-1.
作者信息
Nakano Ryuichi, Nakano Akiyo, Yano Hisakazu, Okamoto Ryoichi
机构信息
Department of Microbiology and Infectious Diseases, Nara Medical University, Kashihara, Nara, Japan.
Department of Microbiology, Kitasato University School of Medicine, Sagamihara, Kanagawa, Japan.
出版信息
mSphere. 2017 Aug 9;2(4). doi: 10.1128/mSphere.00192-17. eCollection 2017 Jul-Aug.
Abstract
CFE-1 is a unique plasmid-encoded AmpC β-lactamase with the regulator gene . It imparts high resistance to most cephalosporins with constitutive high-level β-lactamase activity. Here, the β-lactamase activities and expression levels of with or without were investigated. Results suggested that the resistance of CFE-1 to cephalosporins is caused by a substitution in AmpR, in which the Asp at position 135 is modified to Ala to allow the constitutive high-level expression (derepression) of .
摘要
CFE-1是一种具有调节基因的独特质粒编码AmpCβ-内酰胺酶。它对大多数头孢菌素具有高度耐药性，具有组成型高水平β-内酰胺酶活性。在此，研究了有无（此处原文缺失相关内容）时的β-内酰胺酶活性和表达水平。结果表明，CFE-1对头孢菌素的耐药性是由AmpR中的一个替代引起的，其中第135位的天冬氨酸被修饰为丙氨酸，以允许（此处原文缺失相关内容）的组成型高水平表达（去阻遏）。
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