Suzuki T, Iino T, Horiguchi T, Yamaguchi S
J Bacteriol. 1978 Feb;133(2):904-15. doi: 10.1128/jb.133.2.904-915.1978.
Incomplete flagellar structures were detected in osmotically shocked cells or membrane-associated fraction of many nonflagellate mutants of Salmonella typhimurium by electron microscopy. The predominant types of these structures in the mutants were cistron specific. The incomplete basal bodies were detected in flaFI, flaFIV, flaFVIII, and flaFIX mutants, the structure homologous to a basal body in flaFV mutants, the polyhook-basal body complex in flaR mutants, and the hook-basal body complex in flaL and flaU mutants. No structures homologous to flagellar bases or their parts were detected in the early-fla group nonflagellate mutants of flaAI, flaAII, flaAIII, flaB, flaC, flaD, flaE, flaFII, flaFIII, flaFVI, flaFVII, flaFX, flaK, and flaM. From these observations, a process of flagellar morphogenesis was postulated. The functions of the early-fla group are essential to the formation of S ring-M ring-rod complexes bound to the membrane. The completion of basal bodies requires succeeding functions of flaFI, flaFIV, flaFVIII, and flaFIX. Next, the formation of hooks attached to basal bodies proceeds by the function of flaFV and by flaR, which controls the hook length. Flagellar filaments appear at the tips of hooks because of the functions of flaL, flaU, and flagellin genes.
通过电子显微镜观察发现，在鼠伤寒沙门氏菌许多无鞭毛突变体的渗透休克细胞或膜相关组分中存在不完全鞭毛结构。这些突变体中这些结构的主要类型具有顺反子特异性。在flaFI、flaFIV、flaFVIII和flaFIX突变体中检测到不完全基体，在flaFV突变体中检测到与基体同源的结构，在flaR突变体中检测到多钩-基体复合体，在flaL和flaU突变体中检测到钩-基体复合体。在flaAI、flaAII、flaAIII、flaB、flaC、flaD、flaE、flaFII、flaFIII、flaFVI、flaFVII、flaFX、flaK和flaM的早期fla组无鞭毛突变体中未检测到与鞭毛基部或其部分同源的结构。基于这些观察结果，推测了鞭毛形态发生的过程。早期fla组的功能对于与膜结合的S环-M环-杆复合体的形成至关重要。基体的形成需要flaFI、flaFIV、flaFVIII和flaFIX的后续功能。接下来，附着在基体上的钩的形成通过flaFV的功能以及控制钩长度的flaR进行。由于flaL、flaU和鞭毛蛋白基因的功能，鞭毛丝出现在钩的尖端。
