大肠杆菌K12鞭毛突变体中鞭毛蛋白和钩形亚基蛋白的合成

Synthesis of flagellin and hook subunit protein in flagellar mutants of Escherichia coli K12.

作者信息

Komeda Y, Ono N, Kagawa H

出版信息

Mol Gen Genet. 1984;194(1-2):49-51. doi: 10.1007/BF00383495.

Abstract

We have examined Escherichia coli K12 flagellar mutants affected in each of 29 different loci for the synthesis of flagellin and hook subunit protein. Immune precipitation experiments were employed by treating cell extracts with antiserum against each protein. Flagellin was synthesized in mutants defective in genes flaS , flaT, flaU and flbC . The flaE and flaZ mutants produced small amounts of flagellin, while all the other mutants failed to produce any detectable amount of flagellin. Hook subunit protein was found in most mutants including those defective in genes flaA , flaB , flaC, flaD , flaE , flaG , flaH , flaL , flaM , flaN , flaO , flaP, flaQ , flaR , flaS , flaT, flaU , flaV , flaW , flaX, flaY , flaZ , flbA , flbC , flbD , and hag but not in mutants of flaK , flaI , and flbB . The results conform to the predictions made by our previous indirect gene fusion study ( Komeda 1982).

摘要

我们检测了大肠杆菌K12鞭毛突变体，这些突变体在29个不同位点中的每个位点上，鞭毛蛋白和钩形亚基蛋白的合成均受到影响。通过用针对每种蛋白质的抗血清处理细胞提取物来进行免疫沉淀实验。在flaS、flaT、flaU和flbC基因有缺陷的突变体中合成了鞭毛蛋白。flaE和flaZ突变体产生少量鞭毛蛋白，而所有其他突变体均未能产生任何可检测量的鞭毛蛋白。在大多数突变体中发现了钩形亚基蛋白，包括那些在flaA、flaB、flaC、flaD、flaE、flaG、flaH、flaL、flaM、flaN、flaO、flaP、flaQ、flaR、flaS、flaT、flaU、flaV、flaW、flaX、flaY、flaZ、flbA、flbC、flbD和hag基因有缺陷的突变体，但在flaK、flaI和flbB突变体中未发现。结果与我们之前的间接基因融合研究（小枝田，1982年）所做的预测一致。