Ningde Normal University, Fujian province, China.
The Engineering Technology Research Center of Characteristic Medicinal Plants of Fujian, Ningde Normal University, Fujian province, China.
PLoS One. 2021 Jul 12;16(7):e0254070. doi: 10.1371/journal.pone.0254070. eCollection 2021.
Soft rot disease is a major pathogenic bacteria of Fuding areca taro and has caused serious losses. This study aims to screen biocontrol bacterial against soft rot disease. A total of 53 bacterial strains were isolated from the rhizosphere soil, nine of which exhibited good biocontrol effect against the pathogenic bacteria of soft rot disease as seen in antagonistic screening of biocontrol bacteria from corm in vitro. Strains were selected by physical and chemical experiments, biocontrol effect tests in vivo, molecular sequencing, morphological observation and field tests. Four strains including CAB-L005, CAB-L012, CAB-L014, and CAB-L022 exhibited strong antagonistic effects. On the basis of the sequence homology of 16S rRNA genes, the similarity between strain CAB-L005 and Bacillus tropicus was 100%, that between strain CAB-L012 and Bacillus subtilis was 99%, and that between strain CAB-L014 and Bacillus tequilensis was 100%, and similarity between strain CAB-L022 and Bacillus cereus was 100%. The isolated bacteria demonstrated good biocontrol effects in field experiments. In this study, four strains with good biocontrol application value were isolated and identified, providing a foundation for biocontrol against soft rot disease in areca taro.
软腐病是福鼎槟榔芋的主要病原菌，已造成严重损失。本研究旨在筛选防治软腐病的生防细菌。从根际土壤中分离出 53 株细菌，其中 9 株在离体鳞茎生防细菌拮抗筛选中对软腐病病原菌表现出良好的生防效果。通过理化实验、活体生防效果试验、分子测序、形态观察和田间试验对菌株进行了筛选。包括 CAB-L005、CAB-L012、CAB-L014 和 CAB-L022 在内的 4 株菌株表现出较强的拮抗作用。基于 16S rRNA 基因序列同源性，菌株 CAB-L005 与解淀粉芽孢杆菌的相似度为 100%，菌株 CAB-L012 与枯草芽孢杆菌的相似度为 99%，菌株 CAB-L014 与解淀粉芽孢杆菌的相似度为 100%，菌株 CAB-L022 与蜡样芽孢杆菌的相似度为 100%。分离菌在田间试验中表现出良好的生防效果。本研究分离鉴定了 4 株具有良好生防应用价值的菌株，为槟榔芋软腐病的生物防治提供了依据。
