Li Jieling, Hu Ming, Xue Yang, Chen Xia, Lu Guangtao, Zhang Lianhui, Zhou Jianuan
Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou 510642, China.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, China.
Microorganisms. 2020 May 9;8(5):697. doi: 10.3390/microorganisms8050697.
is the causal agent of bacterial soft rot disease, with a wide range of hosts all over the world. At present, chemical agents, especially agricultural antibiotics, are commonly used in the prevention and control of bacterial soft rot, causing the emergence of resistant pathogens and therefore increasing the difficulty of disease prevention and control. This study aims to provide a safer and more effective biocontrol method for soft rot disease caused by . The spot-on-lawn assay was used to screen antagonistic bacteria, and three strains including SC3, SC11 and 3-10 revealed strong antagonistic effects and were identified as , and , respectively, using multi-locus sequence analysis (MLSA) based on the sequences of 16S rRNA and other housekeeping genes. antimicrobial activity showed that two strains SC3 and SC11 were only antagonistic to some pathogenic bacteria, while strain 3-10 had broad-spectrum antimicrobial activity on both pathogenic bacteria and fungi. Evaluation of control efficacy in greenhouse trials showed that they all restrained the occurrence and development of soft rot disease caused by MS2 or EC1. Among them, strain SC3 had the most impressive biocontrol efficacy on alleviating the soft rot symptoms on both monocotyledonous and dicotyledonous hosts, and strain 3-10 additionally reduced the occurrence of banana wilt disease caused by f. sp. . This is the first report of , and as potential bio-reagents on controlling soft rot disease caused by .
是细菌性软腐病的病原菌，在全球范围内有广泛的寄主。目前，化学药剂，尤其是农用抗生素，常用于细菌性软腐病的防治，导致病原菌产生抗性，进而增加了病害防治的难度。本研究旨在为 引起的软腐病提供一种更安全、更有效的生物防治方法。采用点接菌苔法筛选拮抗细菌，包括SC3、SC11和3-10在内的三株菌株表现出较强的拮抗作用，基于16S rRNA和其他管家基因序列的多位点序列分析（MLSA）分别将它们鉴定为 、 和 。抗菌活性表明，两株 菌株SC3和SC11仅对部分病原菌有拮抗作用，而菌株3-10对病原菌和真菌均有广谱抗菌活性。温室试验中的防效评估表明，它们都能抑制由MS2或EC1引起的软腐病的发生和发展。其中，菌株SC3在减轻单子叶和双子叶寄主上的软腐症状方面具有最显著的生物防治效果，菌株3-10还减少了由 f. sp. 引起的香蕉枯萎病的发生。这是关于 、 和 作为控制由 引起的软腐病的潜在生物试剂的首次报道。
