Neumeister C E
U.S. Department of Health and Human Services, Centers for Disease Control, Cincinnati, OH 45226.
Am Ind Hyg Assoc J. 1987 Nov;48(11):919-21. doi: 10.1080/15298668791385813.
Due to concern over health effects associated with the use of zeranol, a sampling and analytical method to monitor zeranol in the work environment was developed. The collection strategy included air samples on FHLP filters, area samples on gauze pads, hand/dermal samples on gauze pads and bulk samples. The sampling method evaluation included choice of media, flow rate and duration for air samples, sample preparation, extraction efficiencies and sample stability. Analysis of the samples was accomplished by high performance liquid chromatography with identification and quantification by UV and fluorescence detection. The analytical method evaluation included separation of possible interfering compounds, calibration curves, limits of detection (LOD), limits of quantitation (LOQ), precision and accuracy. The tested analytical range was 0.01 to 150 micrograms/mL with a LOD of 0.007 microgram/mL and a LOQ of 0.02 microgram/mL using UV detection. For the fluorescence detector, the LOD was 0.05 microgram/mL and the LOQ was 0.15 microgram/mL. Peak confirmation was achieved by retention time and comparing the two detector responses. Use of this method provided the sensitivity and specificity to analyze environmental samples for zeranol in the workplace environment.
由于担心使用玉米赤霉醇会对健康产生影响，因此开发了一种用于监测工作环境中玉米赤霉醇的采样和分析方法。采集策略包括在FHLP滤膜上采集空气样本、在纱布垫上采集区域样本、在纱布垫上采集手部/皮肤样本以及采集散装样本。采样方法评估包括介质的选择、空气样本的流速和持续时间、样品制备、提取效率和样品稳定性。通过高效液相色谱法对样品进行分析，并通过紫外和荧光检测进行鉴定和定量。分析方法评估包括可能的干扰化合物的分离、校准曲线、检测限（LOD）、定量限（LOQ）、精密度和准确度。使用紫外检测时，测试的分析范围为0.01至150微克/毫升，检测限为0.007微克/毫升，定量限为0.02微克/毫升。对于荧光检测器，检测限为0.05微克/毫升，定量限为0.15微克/毫升。通过保留时间和比较两种检测器的响应来实现峰确认。使用该方法为分析工作场所环境中的玉米赤霉醇环境样本提供了灵敏度和特异性。
